WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem CellsReport as inadecuate




WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells - Download this document for free, or read online. Document in PDF available to download.

Stem Cells International - Volume 2017 2017, Article ID 7848932, 9 pages - https:-doi.org-10.1155-2017-7848932

Research Article

Centre for Neural Engineering, The University of Melbourne, Carlton, VIC 3010, Australia

Department of Psychiatry, The University of Melbourne, Carlton, VIC 3010, Australia

Department of Electrical and Electronic Engineering, The University of Melbourne, Carlton, VIC 3010, Australia

Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, Parkville, VIC, Australia

School of Biomedical Science, Faculty of Medicine and Biomedical Sciences, University of Queensland, St Lucia, QLD 4072, Australia

Department of Anatomy and Neuroscience, The University of Melbourne, Carlton, VIC 3010, Australia

Correspondence should be addressed to Dominic Chi-Hung Ng and Mirella Dottori

Received 21 December 2016; Accepted 22 March 2017; Published 13 June 2017

Academic Editor: Yang D. Teng

Copyright © 2017 Abdullah J.
Alshawaf et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mutations in WD40-repeat protein 62 WDR62 are commonly associated with primary microcephaly and other developmental cortical malformations.
We used human pluripotent stem cells hPSC to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis.
Neurospheres lacking WDR62 expression showed decreased expression of intermediate progenitor marker, TBR2, and also glial marker, S100β.
In contrast, inhibition of c-Jun N-terminal kinase JNK signalling during hPSC neural differentiation induced upregulation of WDR62 with a corresponding increase in neural and glial progenitor markers, PAX6 and EAAT1, respectively.
These findings may signify a role of WDR62 in specifying intermediate neural and glial progenitors during human pluripotent stem cell differentiation.





Author: Abdullah J.
Alshawaf, Ana Antonic, Efstratios Skafidas, Dominic Chi-Hung Ng, and Mirella Dottori


Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents