The response to oestrogen deprivation of the cartilage collagen degradation marker, CTX-II, is unique compared with other markers of collagen turnoverReport as inadecuate




The response to oestrogen deprivation of the cartilage collagen degradation marker, CTX-II, is unique compared with other markers of collagen turnover - Download this document for free, or read online. Document in PDF available to download.

Arthritis Research and Therapy

, 11:R9

First Online: 20 January 2009Received: 24 June 2008Revised: 08 December 2008Accepted: 20 January 2009

Abstract

IntroductionThe urinary level of the type II collagen degradation marker CTX-II is increased in postmenopausal women and in ovariectomised rats, suggesting that oestrogen deprivation induces cartilage breakdown. Here we investigate whether this response to oestrogen is also true for other type II collagen turnover markers known to be affected in osteoarthritis, and whether it relates to its presence in specific areas of cartilage tissue.

MethodsThe type II collagen degradation markers CTX-II and Helix-II were measured in the body fluids of premenopausal and postmenopausal women and in those of ovariectomised rats receiving oestrogen or not. Levels of PIIANP, a marker of type II collagen synthesis, were also measured in rats. Rat knee cartilage was analysed for immunoreactivity of CTX-II and PIIANP and for type II collagen expression.

ResultsAs expected, urinary levels of CTX-II are significantly increased in postmenopausal women and also in oestrogen-deprived rats, although only transiently. However, in neither case were these elevations paralleled by a significant increase of Helix-II levels and PIIANP levels did not change at any time. CTX-II immunoreactivity and collagen expression were detected in different cartilage areas. The upper zone is the area where CTX-II immunoreactivity and collagen expression best reflected the differences in urinary levels of CTX-II measured in response to oestrogen. However, correlations between urinary levels of CTX-II and tissue immunostainings in individual rats were not statistically significant.

ConclusionsWe found only a small effect of oestrogen deprivation on cartilage. It was detected by CTX-II, but not by other type II collagen turnover markers typically affected in osteoarthritis.

AbbreviationsBpbase pair

CTX-IIC-terminal telopeptide of type II collagen

ELISAenzyme-linked immunosorbant assay

HandEhaematoxylin and eosin

OAosteoarthritis

PIIANPpropeptide of type IIA collagen

TBSTris buffered saline.

Electronic supplementary materialThe online version of this article doi:10.1186-ar2596 contains supplementary material, which is available to authorized users.

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Author: Anne-Christine Bay-Jensen - Nadine CB Tabassi - Lene V Sondergaard - Thomas L Andersen - Frederik Dagnaes-Hansen - Patrick

Source: https://link.springer.com/



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