Increased interleukin-23 receptor T cells in peripheral blood mononuclear cells of patients with systemic lupus erythematosusReport as inadecuate




Increased interleukin-23 receptor T cells in peripheral blood mononuclear cells of patients with systemic lupus erythematosus - Download this document for free, or read online. Document in PDF available to download.

Arthritis Research and Therapy

, 12:R215

First Online: 29 November 2010Received: 28 June 2010Revised: 28 October 2010Accepted: 29 November 2010

Abstract

IntroductionSystemic lupus erythematosus SLE is an autoimmune disorder characterized by production of autoantibodies and immune complex deposition in various organs. Aberrations in the T lymphocyte compartment and dysregulated cytokine production are key features of SLE pathogenesis and disease progression. Recently, the role of the interleukin IL-17-IL-23 axis in the pathogenesis of SLE has been reported. IL-23 and IL-23R are essential for expansion of pathogenic IL-17-producing T lymphocytes and have been shown to be important in the pathogenesis of lupus in animal models.

MethodsIn this study, the expression of IL-23R and IL-17 in CD4 and CD8 T lymphocytes in peripheral blood mononuclear cells PBMCs of SLE patients and control subjects were examined by flow cytometry. Twenty-nine SLE patients and 10 control subjects were recruited in this study. Patients were divided into active and inactive groups based on the SLE disease activity index SLEDAI. As another disease control population, five psoriatic patients were recruited in this study.

ResultsPercentages of both IL23R CD4 and IL-23R CD8 T cell subsets were significantly higher in freshly isolated PBMCs from both groups of SLE patients compared to control subjects P = 0.0021 and P = 0.0006, respectively. In addition, this difference was maintained after ex vivo stimulation with plate-bound anti-CD3-CD28 antibodies P = 0.007 and P = 0.0019, respectively. When the fold increase in IL-17 T cells after ex vivo stimulation for three days was compared between patients and controls, SLE patients exhibited significantly higher increases in CD4 IL-17 and CD8 IL-17 T cells, suggesting that PBMCs from SLE patients promoted the expansion of IL-17-producing T cells upon stimulation more vigorously than control PBMCs. These trends were not observed in psoriasis patients. The correlations between IL-23R T cells and IL-17 T cells and IL-23R CD8 T cells and SLEDAI scores in patients were also found to be statistically significant.

ConclusionsThe results of our study confirmed the relevance of the IL-23-IL-17 axis in the pathogenesis of SLE and further highlighted the importance of IL-23R T cell subsets in this autoimmune disease.

AbbreviationsILinterleukin

PMBCsperipheral blood mononuclear cells

SLEsystemic lupus erythematosus

SLEDAISLE disease activity index.

Electronic supplementary materialThe online version of this article doi:10.1186-ar3194 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Author: Hathaipat Puwipirom - Nattiya Hirankarn - Pimpayao Sodsai - Yingyos Avihingsanon - Jongkonnee Wongpiyabovorn - Tanapat Palag

Source: https://link.springer.com/



DOWNLOAD PDF




Related documents