Quantitative metabolic profiles of 2nd and 3rd trimester human amniotic fluid using 1H HR-MAS spectroscopyReport as inadecuate




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Magnetic Resonance Materials in Physics, Biology and Medicine

, 22:343

First Online: 25 September 2009Received: 15 April 2009Revised: 21 August 2009Accepted: 08 September 2009

Abstract

ObjectTo establish and compare normative metabolite concentrations in 2nd and 3rd trimester human amniotic fluid samples in an effort to reveal metabolic biomarkers of fetal health and development.

Materials and methodsTwenty-one metabolite concentrations were compared between 2nd 15–27 weeks gestation, N = 23 and 3rd 29–39 weeks gestation, N = 27 trimester amniotic fluid samples using H high resolution magic angle spinning HR-MAS spectroscopy. Data were acquired using the electronic reference to access in vivo concentrations method and quantified using a modified semi-parametric quantum estimation algorithm modified for high-resolution ex vivo data.

ResultsSixteen of 21 metabolite concentrations differed significantly between 2nd and 3rd trimester groups. Betaine 0.00846±0.00206 mmol-kg vs. 0.0133±0.0058 mmol-kg, P < 0.002 and creatinine 0.0124±0.0058 mmol-kg vs. 0.247±0.011 mmol-kg, P < 0.001 concentrations increased significantly, while glucose 5.96±1.66 mmol-kg vs. 2.41±1.69 mmol-kg, P < 0.001, citrate 0.740±0.217 mmol-kg vs. 0.399±0.137 mmol-kg, P < 0.001, pyruvate 0.0659±0.0103 mmol-kg vs. 0.0299±0.286 mmol-kg, P < 0.001, and numerous amino acid e.g. alanine, glutamate, isoleucine, leucine, lysine, and valine concentrations decreased significantly with advancing gestation. A stepwise multiple linear regression model applied to 50 samples showed that gestational age can be accurately predicted using combinations of alanine, glucose and creatinine concentrations.

ConclusionThese results provide key normative data for 2nd and 3rd trimester amniotic fluid metabolite concentrations and provide the foundation for future development of magnetic resonance spectroscopy MRS biomarkers to evaluate fetal health and development.

KeywordsElectronic reference to access in vivo concentrations ERETIC Gestational age High-resolution magic angle spinning HR-MAS Spectroscopy High-resolution quantum estimation HR-QUEST Human amniotic fluid  Download to read the full article text



Author: Brad R. Cohn - Bonnie N. Joe - Shoujun Zhao - John Kornak - Vickie Y. Zhang - Rahwa Iman - John Kurhanewicz - Kiarash V

Source: https://link.springer.com/







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