Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein ContentReport as inadecuate




Undernourishment and Yersinia enterocolitica Enterocolitis Alter Intestinal Contractility in the Rabbit: Role of Smooth Muscle Contractile Protein Content - Download this document for free, or read online. Document in PDF available to download.

Canadian Journal of Gastroenterology - Volume 13 1999, Issue 4, Pages 319-324

Original Article Gastrointestinal Research Group and the Department of Pediatrics, University of Calgary, Calgary, Alberta, Canada



Copyright © 1999 Hindawi Publishing Corporation. This open-access article is distributed under the terms of the Creative Commons Attribution Non-Commercial License CC BY-NC http:-creativecommons.org-licenses-by-nc-4.0-, which permits reuse, distribution and reproduction of the article, provided that the original work is properly cited and the reuse is restricted to noncommercial purposes.

Abstract

Previous studies have demonstrated that the longitudinal smooth muscle of rabbits infected with Yersinia enterocolitica and undernourished because of reduced food intake exhibit a significantly reduced ability to develop tension in response to carbachol compared with pair-fed animals, which are uninfected but equivalently undernourished. To determine whether the alteration in smooth muscle contractility results from changes in cell number hypo- or hyperplasia, or in contractile protein content or isoform distribution, New Zealand White rabbits 600 to 1000 g were randomly assigned to one of three treatment groups: infected, pair-fed or control. Tissue contractility was measured, morphometric studies were performed and immunoassays were developed for the measurement of total actin, gamma-enteric and alpha-vascular isoactins, and myosin heavy chain. Consistent with what was found in previous reports, the contractility of longitudinal smooth muscle in response to carbachol was found to increase in pair-fed animals and to decrease in Y enterocolitica-infected animals. There was no significant change in the proportional thickness of the ileal longitudinal smooth muscle coat, and the number of cross-sectioned longitudinal smooth muscle cells-mm

was not significantly different in infected, pair-fed or control tissues. Immunoassay indicated that the proportion of each specific contractile protein, relative to total protein content in the muscularis propria, was unaffected by Y enterocolitica infection or by pair-feeding. Thus, the alterations in intestinal longitudinal smooth muscle function observed after Y enterocolitica infection were concluded not to be associated with tissue hypo- or hyperplasia, or changes in the total content or isoform distribution of contractile proteins in the muscularis propria.





Author: Beth C Chin, Daimen TM Tan, and R Brent Scott

Source: https://www.hindawi.com/



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