Effect of in uteroexposure to diethylstilbestrol on lumbar and femoral bone, articular cartilage, and the intervertebral disc in male and female adult mice progeny with and without swimming exercise Report as inadecuate




Effect of in uteroexposure to diethylstilbestrol on lumbar and femoral bone, articular cartilage, and the intervertebral disc in male and female adult mice progeny with and without swimming exercise - Download this document for free, or read online. Document in PDF available to download.

Arthritis Research and Therapy

, 14:R17

First Online: 23 January 2012Received: 26 April 2011Accepted: 23 January 2012

Abstract

IntroductionDevelopmental exposure to estrogens has been shown to affect the musculoskeletal system. Furthermore, recent studies have shown that environmental exposure to estrogen-like compounds is much higher than originally anticipated. The aim of this study was to determine the effects of diethylstilbestrol DES, a well-known estrogen agonist, on articular cartilage, intervertebral disc IVD, and bone phenotype.

MethodsC57Bl-6 pregnant mice were dosed orally with vehicle peanut oil or 0.1, 1.0, and 10 μg-kg-day of DES on gestational days 11 to 14. Male and female pups were allowed to mature without further treatment until 3 months of age, when swim and sedentary groups were formed. After euthanasia, bone mineral density BMD, bone mineral content BMC, bone area BA, and trabecular bone area TBA of the lumbar vertebrae and femur were measured by using a PIXImus Bone Densitometer System. Intervertebral disc proteoglycan was measured with the DMMB assay. Histologic analysis of proteoglycan for IVD and articular cartilage was performed with safranin O staining, and degeneration parameters were scored.

ResultsThe lumbar BMC was significantly increased in female swimmers at both the highest and lowest dose of DES, whereas the femoral BMC was increased only at the highest. The males, conversely, showed a decreased BMC at the highest dose of DES for both lumbar and femoral bone. The female swim group had an increased BA at the highest dose of DES, whereas the male counterpart showed a decreased BA for femoral bone. The TBA showed a similar pattern. Proteoglycan analysis of lumbar IVDs showed a decrease at the lowest doses but a significant increase at the highest doses for both males and females. Histologic examination showed morphologic changes of the IVD and articular cartilage for all doses of DES.

ConclusionsDES significantly affected the musculoskeletal system of adult mice. Results suggest that environmental estrogen contaminants can have a detrimental effect on the developmental lumbar bone growth and mineralization in mice. Further studies measuring the impact of environmental estrogen mimics, such as bisphenol A, are then warranted.

AbbreviationsAFannulus fibrosus

BAbone area

BMCbone mineral content

BMDbone mineral density

CTcomputed tomography

DESdiethylstilbestrol

ERestrogen receptor

GAGproteoglycan

IVDintervertebral disc

NPnucleus pulposus

TBAtrabecular bone area.

Electronic supplementary materialThe online version of this article doi:10.1186-ar3696 contains supplementary material, which is available to authorized users.

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Author: Sora Al Rowas - Rami Haddada - Rahul Gawri - Abdul Aziz Al Ma-awi - Lorraine E Chalifour - John Antoniou - Fackson Mwale

Source: https://link.springer.com/







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