Cytotoxic and Apoptotic Effects of Different Extracts of Artemisia turanica Krasch. on K562 and HL-60 Cell LinesReport as inadecuate




Cytotoxic and Apoptotic Effects of Different Extracts of Artemisia turanica Krasch. on K562 and HL-60 Cell Lines - Download this document for free, or read online. Document in PDF available to download.

The Scientific World JournalVolume 2013 2013, Article ID 628073, 6 pages

Research Article

Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 9188617871, Iran

Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad 9188617871, Iran

Novel Drug Delivery Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah 6734667149, Iran

Received 23 August 2013; Accepted 11 September 2013

Academic Editors: J. M. Nesland and D. Noonan

Copyright © 2013 Zahra Tayarani-Najaran et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Artemisia is an important genus of Iranian flora. Cytotoxic activities for some species of the genus have already been reported. In this study, we have investigated the cytotoxic effects of n-hexane, CH2Cl2, EtOAc, EtOH, and EtOH-H2O 1 : 1 extracts of A. turanica Krasch. on two human leukemic cancer cell lines K562 and HL-60 and J774 as normal cells using alamarBlue resazurin assay. PI staining of the fragmented DNA and western blot analysis were used to evaluate the possible apoptotic effect of the extract. The CH2Cl2 extract of A. turanica showed the most antiproliferative effect on cancer cells among all tested extracts with IC50 values of 69 and 104 μg-mL on K562 and HL-60 cells, respectively, whereas the normal cells were not affected significantly by this extract. Sub-G1 peak in the flow cytometry histogram of the cells treated with CH2Cl2 extract of A. turanica and cleavage of PARP protein confirmed the induction of apoptosis with CH2Cl2 extract. Taken together, the findings of the present work suggest the anticancer potential of CH2Cl2 extract of A. turanica on human leukemic cancer cell lines.





Author: Zahra Tayarani-Najaran, Mahla Sareban, Atefeh Gholami, Seyed Ahmad Emami, and Mahdi Mojarrab

Source: https://www.hindawi.com/



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