Rapid assessment of myocardial infarct size in rodents using multi-slice inversion recovery late gadolinium enhancement CMR at 9.4TReport as inadecuate




Rapid assessment of myocardial infarct size in rodents using multi-slice inversion recovery late gadolinium enhancement CMR at 9.4T - Download this document for free, or read online. Document in PDF available to download.

Journal of Cardiovascular Magnetic Resonance

, 13:44

First Online: 05 September 2011Received: 12 April 2011Accepted: 05 September 2011

Abstract

BackgroundMyocardial infarction MI can be readily assessed using late gadolinium enhancement LGE cardiovascular magnetic resonance CMR. Inversion recovery IR sequences provide the highest contrast between enhanced infarct areas and healthy myocardium. Applying such methods to small animals is challenging due to rapid respiratory and cardiac rates relative to T1 relaxation.

MethodsHere we present a fast and robust protocol for assessing LGE in small animals using a multi-slice IR gradient echo sequence for efficient assessment of LGE. An additional Look-Locker sequence was used to assess the optimum inversion point on an individual basis and to determine most appropriate gating points for both rat and mouse. The technique was applied to two preclinical scenarios: i an acute 2 hour reperfused model of MI in rats and ii mice 2 days following non-reperfused MI.

ResultsLGE images from all animals revealed clear areas of enhancement allowing for easy volume segmentation. Typical inversion times required to null healthy myocardium in rats were between 300-450 ms equivalent to 2-3 R-waves and ~330 ms in mice, typically 3 R-waves following inversion. Data from rats was also validated against triphenyltetrazolium chloride staining and revealed close agreement for infarct size.

ConclusionThe LGE protocol presented provides a reliable method for acquiring images of high contrast and quality without excessive scan times, enabling higher throughput in experimental studies requiring reliable assessment of MI.

Electronic supplementary materialThe online version of this article doi:10.1186-1532-429X-13-44 contains supplementary material, which is available to authorized users.

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Author: Anthony N Price - King K Cheung - Shiang Y Lim - Derek M Yellon - Derek J Hausenloy - Mark F Lythgoe

Source: https://link.springer.com/article/10.1186/1532-429X-13-44







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