Activation of TRPV1 Prevents OxLDL-Induced Lipid Accumulation and TNF-α-Induced Inflammation in Macrophages: Role of Liver X Receptor αReport as inadecuate




Activation of TRPV1 Prevents OxLDL-Induced Lipid Accumulation and TNF-α-Induced Inflammation in Macrophages: Role of Liver X Receptor α - Download this document for free, or read online. Document in PDF available to download.

Mediators of InflammationVolume 2013 2013, Article ID 925171, 14 pages

Research Article

Department of Physiology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan

Division of Cardiology, Department of Internal Medicine, Taipei Veterans General Hospital, Taipei 11221, Taiwan

Heart Center, Cheng-Hsin General Hospital, Taipei 11221, Taiwan

Cardiovascular Division, Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan

Received 15 April 2013; Accepted 26 May 2013

Academic Editor: Ishak Tekin

Copyright © 2013 Jin-Feng Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The transient receptor potential vanilloid type 1 TRPV1 is crucial in the pathogenesis of atherosclerosis; yet its role and underlying mechanism in the formation of macrophage foam cells remain unclear. Here, we show increased TRPV1 expression in the area of foamy macrophages in atherosclerotic aortas of apolipoprotein E-deficient mice. Exposure of mouse bone-marrow-derived macrophages to oxidized low-density lipoprotein oxLDL upregulated the expression of TRPV1. In addition, oxLDL activated TRPV1 and elicited calcium Ca

influx, which were abrogated by the pharmacological TRPV1 antagonist capsazepine. Furthermore, oxLDL-induced lipid accumulation in macrophages was ameliorated by TRPV1 agonists but exacerbated by TRPV1 antagonist. Treatment with TRPV1 agonists did not affect the internalization of oxLDL but promoted cholesterol efflux by upregulating the efflux ATP-binding cassette ABC transporters ABCA1 and ABCG1. Moreover, the upregulation of ABC transporters was mainly through liver X receptor α- LXRα- dependent regulation of transcription. Moreover, the TNF-α-induced inflammatory response was alleviated by TRPV1 agonists but aggravated by the TRPV1 antagonist and LXRα siRNA in macrophages. Our data suggest that LXRα plays a pivotal role in TRPV1-activation-conferred protection against oxLDL-induced lipid accumulation and TNF-α-induced inflammation in macrophages.





Author: Jin-Feng Zhao, Li-Chieh Ching, Yu Ru Kou, Shing-Jong Lin, Jeng Wei, Song-Kun Shyue, and Tzong-Shyuan Lee

Source: https://www.hindawi.com/



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