Long-term persistence and effects of fetal microchimerisms on disease onset and status in a cohort of women with rheumatoid arthritis and systemic lupus erythematosusReport as inadecuate




Long-term persistence and effects of fetal microchimerisms on disease onset and status in a cohort of women with rheumatoid arthritis and systemic lupus erythematosus - Download this document for free, or read online. Document in PDF available to download.

BMC Musculoskeletal Disorders

, 14:325

Clinical rheumatology and osteoporosis

Abstract

BackgroundThe discovery of a fetal cells transfer to the mother is a phenomenon with multiple implications for autoimmunity and tolerance. The prevalence and meaning of the feto-maternal microchimerism MC in rheumatic diseases has not been thoroughly investigated. The aim of this study was to analyze the prevalence of fetal MC in patients with inflammatory rheumatic diseases and to investigate the association of MC with disease onset and current status.

MethodsA total of 142 women who gave birth to at least one male offspring were recruited: 72 women with rheumatoid arthritis RA, 16 women with systemic lupus erythematosus SLE, and 54 healthy women. For the detection of fetal microchimerism a nested PCR method was used to amplify a Y chromosome specific sequence TSPY1. For characterization of disease activity we analyzed autoantibody profiles and X-rays in RA, and in addition complement levels in SLE respectively.

ResultsA significant higher prevalence of fetal MC was found in RA 18% and SLE 31% compared to controls 3.7% p = 0.02 and p = 0.006, resp

The mean age at disease onset was comparable in MC + and MC- RA patients. Disease onset occurred 18.7 MC + and 19.8 MC- years post partum of the first son, respectively. The presence of anti-CCP and RF did not differ significantly, anti-CCP were found in 75% of MC + and 87% of MC- patients, RF in 75% of both MC + and MC- patients. A slightly higher mean Steinbrocker score in MC + patients was associated with longer disease duration in MC + compared to MC- RA. In SLE patients the mean age at disease onset was 42.6 years in MC + and 49.1 years in MC- patients. Disease onset occurred 24.0 and 26.4 years post partum of the first son for MC + and MC- patients, respectively. The presence of ANA and anti-dsDNA antibodies, C3, C4 and CH50 did not differ significantly.

ConclusionOur results indicate a higher frequency of long-term male MC in RA and SLE patients compared with controls without impact on disease onset and status in RA and SLE.

KeywordsMicrochimerism RA SLE Pregnancy AbbreviationsANAAnti-nuclear antibody

CCPCyclic citrullinated peptide

FISHFluorescence in situ hybridization

HCHealthy controls

JIAJuvenile idiopathic arthritis

MCMicrochimerism

PCRPolymerase chain reaction

RARheumatoid arthritis

SLESystemic lupus erythematosus

TE bufferTris-EDTA buffer

TSPY1Testis specific protein Y-linked 1

yrsYears.

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Author: Marianne Kekow - Maria Barleben - Susanne Drynda - Sibylle Jakubiczka - Jörn Kekow - Thomas Brune

Source: https://link.springer.com/



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