Cellular Signal Transduction Pathways by Leptin in Colorectal Cancer Tissue: Preliminary ResultsReport as inadecuate




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ISRN EndocrinologyVolume 2011 2011, Article ID 575397, 4 pages

Research Article

Department of Internal Diseases, Medical University of Silesia, Żeromskiego 7 Street, 41-902 Bytom, Poland

Department of Molecular Biology, Medical University of Silesia, Narcyzów 1 Street, 41-200 Sosnowiec, Poland

Department of Human Nutrition, Medical University of Silesia, Piekarska 18 Street, 41-902 Bytom, Poland

Received 17 December 2010; Accepted 27 January 2011

Academic Editor: M. Tambascia

Copyright © 2011 Ewa Nowakowska-Zajdel et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the study was to analyse genes typing with the use of the oligonucleotide microarray technique HG-U133A, Affymetrix differentiating colorectal cancer tissues from tissues assessed histopathologically as healthy ones among a panel of 91 mRNA of genes encoding proteins involved in activation of cellular signal transduction pathways by leptin.
Frozen tumor specimens from 11 colon cancer patients in various stages of clinical progression of the disease in an I–IV stage scale according to the TNM staging were used in molecular tests.
Among the genes participating in the cascade of signal transfer in cell activated by leptin, the following ones: AKT1, STAT3, MCL1 were qualified as differentiating stage I and II and VEGFC, CCNDI the encoding genes respectively as differentiating III and IV stage neoplasm.
It is necessary to extend studies of analysis of cellular signal transduction pathways by leptin in colorectal cancer initiation and transformation processes.





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Source: https://www.hindawi.com/



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