Association of large intergenic noncoding RNA expression with disease activity and organ damage in systemic lupus erythematosusReport as inadecuate




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Arthritis Research and Therapy

, 17:131

First Online: 21 May 2015Received: 29 November 2014Accepted: 20 April 2015

Abstract

IntroductionDespite growing evidence that large intergenic noncoding RNAs lincRNAs can regulate gene expression and widely take part in normal physiological and disease conditions, our knowledge of systemic lupus erythematosus SLE-related lincRNAs remains limited. The aim of this study was to detect the levels of four lincRNAs ENST00000500949: linc0949, ENST00000500597: linc0597, ENST00000501992: linc1992, and ENST00000523995: linc3995 involved in innate immunity in the peripheral blood mononuclear cells PBMCs of patients with SLE and correlate these lincRNA levels with disease activity, organ damage, clinical features and medical therapies.

MethodsPBMCs were obtained from 102 patients with SLE, 54 patients with rheumatoid arthritis RA and 76 healthy donors. lincRNA expression levels were measured by real-time quantitative polymerase chain reaction. Disease activity was assessed using the Systemic Lupus Erythematosus Disease Activity Index 2000 SLEDAI-2K scores, and organ damage was evaluated with the Systemic Lupus International Collaborating Clinics-American College of Rheumatology Damage Index.

Resultslinc0949 and linc0597 were significantly decreased in patients with SLE compared with patients with RA and healthy control subjects. linc0949 was correlated with SLEDAI-2K score r = −0.329, P = 0.0007, as well as with complement component C3 level r = 0.348, P = 0.0003. The level of linc0949 was also reduced in patients with SLE who had the presence of cumulative organ damage. In addition, decreasing expression of linc0949 was associated with lupus nephritis. linc0949 expression significantly increased after treatment, whereas neither disease activity nor organ damage correlated with linc0597 expression.

ConclusionsOur results provide novel empirical evidence that linc0949 could be a potential biomarker for diagnosis, disease activity and therapeutic response in SLE.

AbbreviationsACRAmerican College of Rheumatology

ANAAntinuclear antibody

AZAAzathioprine

C3Complement component 3

CsACyclosporine A

CYCCyclophosphamide

dsDNADouble-stranded DNA

DXMDexamethasone

EDTAEthylenediaminetetraacetic acid

FBSFetal bovine serum

FK506Tacrolimus

GAS5Growth arrest–specific 5

hpfHigh-power field

IFNInterferon

ILInterleukin

LEFLeflunomide

linc0597ENST00000500597

linc0949ENST00000500949

linc1992ENST00000501992

linc3995ENST00000523995

lincRNALarge intergenic noncoding RNA

LNLupus nephritis

lncRNALong noncoding RNA

miRNAmicroRNA

MMFMycophenolate mofetil

MTXMethotrexate

NSNot significant

ntNucleotide

PBMCPeripheral blood mononuclear cell

RARheumatoid arthritis

RNPRibonucleoprotein

RPL13ARibosomal protein L13A gene

RT-qPCRReal-time quantitative polymerase chain reaction

SDISystemic Lupus International Collaborating Clinics-American College of Rheumatology Damage Index

SEMStandard error of the mean

SLESystemic lupus erythematosus

SLEDAI-2KSystemic Lupus Erythematosus Disease Activity Index 2000

SLICCSystemic Lupus International Collaborating Clinics

SmSmith

SSASjögren’s syndrome–related antigen A

SSBSjögren’s syndrome–related antigen B

TLRToll-like receptor

TNFTumor necrosis factor

SEMStandard error of the mean

Electronic supplementary materialThe online version of this article doi:10.1186-s13075-015-0632-3 contains supplementary material, which is available to authorized users.

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Author: Yanfang Wu - Feifei Zhang - Jianyang Ma - Xiaoyan Zhang - Lingling Wu - Bo Qu - Shiwei Xia - Shunle Chen - Yuanjia Tang -

Source: https://link.springer.com/



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