Bacterial Community Profile of the Gut Microbiota Differs between Hypercholesterolemic Subjects and ControlsReport as inadecuate




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BioMed Research International - Volume 2017 2017, Article ID 8127814, 6 pages - https:-doi.org-10.1155-2017-8127814

Research Article

Center of Molecular Biology and Pharmacogenetics, Department of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile

Department of Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile

Applied Microbial Ecology Laboratory, Department of Chemical Sciences and Natural Resources, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco, Chile

Faculty of Health, Universidad Santo Tomás, Temuco, Chile

Correspondence should be addressed to Nicolás Saavedra

Received 13 March 2017; Revised 18 May 2017; Accepted 23 May 2017; Published 15 June 2017

Academic Editor: Clara G. de los Reyes-Gavilan

Copyright © 2017 Camilo Rebolledo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The role of gut microbiota in the development of metabolic illnesses has been abundantly demonstrated. Recent studies suggest that gut microbiota alterations may also be related to the development of hypercholesterolemia. Therefore, we aimed to assess differences in the gut bacterial community profiles between hypercholesterolemic subjects and controls. Thirty cases diagnosed with hypercholesterolemia and 27 normocholesterolemic controls were included. A fasting whole blood sample was obtained to determine the lipid profile. In parallel, stool samples were collected and total DNA was isolated to assess the bacterial community profiles by denaturing gradient gel electrophoresis DGGE. In addition, the Richness, Shannon-Weaver, and Simpson indexes were used to evaluate the richness and diversity of bacterial communities. As expected, serum concentrations of total cholesterol, triglycerides, and LDL-cholesterol were significantly higher in the cases compared with controls. Moreover, DGGE analysis showed a lower richness and diversity of bacterial communities in hypercholesterolemic subjects. In conclusion, our results showed differences in the profiles of bacterial communities between hypercholesterolemic subjects and controls, suggesting a possible role of the gut microbiota in the development of hypercholesterolemia.





Author: Camilo Rebolledo, Alejandro Cuevas, Tomás Zambrano, Jacquelinne J. Acuña, Milko A. Jorquera, Kathleen Saavedra, Claudia Ma

Source: https://www.hindawi.com/



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