Optical Imaging of Tumor Response to Hyperbaric Oxygen Treatment and Irradiation in an Orthotopic Mouse Model of Head and Neck Squamous Cell CarcinomaReport as inadecuate




Optical Imaging of Tumor Response to Hyperbaric Oxygen Treatment and Irradiation in an Orthotopic Mouse Model of Head and Neck Squamous Cell Carcinoma - Download this document for free, or read online. Document in PDF available to download.

Molecular Imaging and Biology

, Volume 17, Issue 5, pp 633–642

First Online: 28 February 2015

Abstract

PurposeHyperbaric oxygen therapy HBOT is used in the treatment of radiation-induced tissue injury but its effect on residual tumor tissue is indistinct and therefore investigated in this study.

ProceduresOrthotopic FaDu tumors were established in mice, and the response of the irradiated tumors to HBOT was monitored by bioluminescence imaging. Near infrared fluorescence imaging using AngioSense750 and Hypoxisense680 was applied to detect tumor vascular permeability and hypoxia.

ResultsHBOT treatment resulted in accelerated growth of non-irradiated tumors, but mouse survival was improved. Tumor vascular leakiness and hypoxia were enhanced after HBOT, whereas histological characteristics, epithelial-to-mesenchymal transition markers, and metastatic incidence were not influenced.

ConclusionsSquamous cell carcinoma responds to HBOT with respect to tumor growth, vascular permeability, and hypoxia, which may have implications for its use in cancer patients. The ability to longitudinally analyze tumor characteristics highlights the versatility and potential of optical imaging methods in oncological research.

Key wordsHyperbaric oxygen therapy Radiation Optical imaging Near infrared fluorescence Squamous cell carcinoma Lymph node metastasis Animal model Head and neck cancer Electronic supplementary materialThe online version of this article doi:10.1007-s11307-015-0834-8 contains supplementary material, which is available to authorized users.

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Author: Joanna A. M. Braks - Linda Spiegelberg - Senada Koljenovic - Yanto Ridwan - Stijn Keereweer - Roland Kanaar - Eppo B. Wo

Source: https://link.springer.com/







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