In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled SiderophoresReport as inadecuate




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Molecular Imaging and Biology

, Volume 18, Issue 3, pp 344–352

First Online: 30 September 2015

Abstract

PurposeSome Gasiderophores show promise in specific and sensitive imaging of infection. Here, we compare the in vitro and in vivo behaviour of selected Ga-68 and Zr-89 labelled siderophores.

ProceduresRadiolabelling was performed in HEPES or sodium acetate buffer systems. Radiochemical purity of labelled siderophores was determined using chromatography. Partition coefficients, in vitro stability and protein binding affinities were determined. Ex vivo biodistribution and animal imaging was studied in mice.

ResultsCertain differences among studied siderophores were observed in labelling efficiency. Protein binding and stability tests showed highest stabilities and lowest protein binding affinities for Ga-68 and Zrtriacetylfusarinine C TAFC. All studied Ga-68 and Zrsiderophores exhibited a similar biodistribution and pharmacokinetics in mice with the exception of Zrferrioxamine E FOXE.

ConclusionsZr-89 and Gasiderophores showed analogous in vitro and in vivo behaviour. Tested Zrsiderophores could be applied for longitudinal positron emission tomography PET studies of fungal infections and especially TAFC for the development of novel bioconjugates.

Key wordsSiderophores Gallium-68 Zirconium-89 PET Imaging Electronic supplementary materialThe online version of this article doi:10.1007-s11307-015-0897-6 contains supplementary material, which is available to authorized users.

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Author: Milos Petrik - Chuangyan Zhai - Zbynek Novy - Lubor Urbanek - Hubertus Haas - Clemens Decristoforo

Source: https://link.springer.com/



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