Impaired coronary microcirculation in type 2 diabetic patients is associated with elevated circulating regulatory T cells and reduced number of IL-21R T cellsReport as inadecuate




Impaired coronary microcirculation in type 2 diabetic patients is associated with elevated circulating regulatory T cells and reduced number of IL-21R T cells - Download this document for free, or read online. Document in PDF available to download.

Cardiovascular Diabetology

, 15:67

First Online: 19 April 2016Received: 20 January 2016Accepted: 25 March 2016

Abstract

BackgroundLow-grade systemic inflammation is considered to participate in the progression of type 2 diabetes T2D and in diabetic complications.

MethodsTo determine if circulating leukocytes were abnormally regulated in T2D patients, 8-color flow-cytometry FACS analysis was performed in a cross-sectional study of 37 T2D patients and 16 controls. Data obtained from the FACS analysis were compared to coronary flow reserve CFR, assessed by Rb-PET-imaging, to uncover inflammatory signatures associated with impaired CFR.

ResultsPresence of T2D was associated with T cell attenuation characterized by reduced overall T cell, Th17, IL-21R, Treg’s and TLR4 T cells, while the monocyte population showed enhanced TLR4 expression. Further, our data revealed reduced M1-like CD11c expression in T2D which was associated with impaired CFR. In contrast, we show, for the first time in T2D, increased TLR4 expression on CD8 T cells, increased Treg cell number and Treg maturation and reduced IL-21R expression on CD8 T cells to be functionally associated with impaired CFR.

ConclusionsOur demonstration that HbA1c inversely correlates to several T cell populations suggests that T cells may play disease modulating roles in T2D. Further, the novel association between impaired CFR and regulatory T cells and IL-21R T cells imply an intricate balance in maintaining tissue homeostasis in vascular diabetic complications.

KeywordsType 2 diabetes Flow-cytometry FACS analysis Coronary microcirculation Coronary flow reserve Inflammation Cardiovascular disease Peripheral blood Monocyte sub-populations Bernt Johan von Scholten and Alexander Rosendahl contributed equally to this work

Electronic supplementary materialThe online version of this article doi:10.1186-s12933-016-0378-5 contains supplementary material, which is available to authorized users.

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Author: Bernt Johan von Scholten - Alexander Rosendahl - Philip Hasbak - Regine Bergholdt - Andreas Kjaer - Peter Rossing - Tine W

Source: https://link.springer.com/article/10.1186/s12933-016-0378-5



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