PCR-SSCP Variation of IGF1 and PIT1 Genes and Their Association with Estimated Breeding Values of Growth Traits in Makooei SheepReport as inadecuate




PCR-SSCP Variation of IGF1 and PIT1 Genes and Their Association with Estimated Breeding Values of Growth Traits in Makooei Sheep - Download this document for free, or read online. Document in PDF available to download.

Genetics Research InternationalVolume 2013 2013, Article ID 272346, 6 pages

Research Article

Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran

Young Researchers and Elites Club, Khorasgan Branch, Islamic Azad University, Isfahan, Iran

Received 1 July 2013; Revised 26 October 2013; Accepted 30 October 2013

Academic Editor: Michael Walter

Copyright © 2013 Masoud Negahdary et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Molecular biology techniques genetic improvement by facilitating identification, mapping and analysis of polymorphism of genes by encoding proteins that act on metabolic pathways involved in economically interesting traits. This use of genetic markers can aid identification of those animals with the highest breeding values in sheep. On the basis of sheep genome mapping, information was examined on the ovine IGF1 and PIT1 genes as a possible genetic marker for growth traits in sheep. The current study was designed to estimate the frequencies of putative IGF-1 and PIT-1 genes SNPs and investigate associations with calculated EBVs of growth traits in Makooei sheep. PCR-SSCP analysis of the exon1 of IGF-I gene and include a part of intron2, exon3 and a part of intron3 and PIT-1 gene revealed the following banding patterns; three AA, AG, GG and four AA p1, AB p2, CC p3, CD p4, banding patterns respectively. Results from this study demonstrated higher performance of AA animals in BW and GBW, and AG animal in WW and W6 that may be related to the role of IGF-1 at the pre-puberty and puberty stages. Also higher performance of p3 animals in W9, YW and GSN, and p1 animal in GNY may be related to the PIT-1 role in post-puberty.





Author: Masoud Negahdary, Abbas Hajihosseinlo, and Marziyeh Ajdary

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents