Integrated Treatment of Aqueous Extract of Solanum nigrum-Potentiated Cisplatin- and Doxorubicin-Induced Cytotoxicity in Human Hepatocellular Carcinoma CellsReport as inadecuate




Integrated Treatment of Aqueous Extract of Solanum nigrum-Potentiated Cisplatin- and Doxorubicin-Induced Cytotoxicity in Human Hepatocellular Carcinoma Cells - Download this document for free, or read online. Document in PDF available to download.

Evidence-Based Complementary and Alternative Medicine - Volume 2015 2015, Article ID 675270, 9 pages -

Research Article

Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan

Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan

Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan

Department of Chinese Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan

Division of General Surgery, Department of Surgery, Chi-Mei Medical Center, Tainan 710, Taiwan

Department of Obstetrics and Gynecology, Taipei Medical University Hospital, Taipei 110, Taiwan

Cancer Research Center, Taipei Medical University and Hospital, Taipei 110, Taiwan

Department of Surgery, Taipei Medical University and Hospital, Taipei 110, Taiwan

Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei Medical University, Taipei 110, Taiwan

Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan

Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan

Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 110, Taiwan

Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan

Received 26 September 2014; Revised 26 December 2014; Accepted 26 December 2014

Academic Editor: Mohammad Ahmad Al-Shatouri

Copyright © 2015 Chien-Kai Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chemotherapy is the main approach for treating advanced and recurrent hepatocellular carcinoma HCC, but the clinical performance of chemotherapy is limited by a relatively low response rate, drug resistance, and adverse effects that severely affect the quality of life of patients. The aqueous extract of Solanum nigrum AE-SN is a crucial ingredient in some traditional Chinese medicine TCM formulas for treating cancer patients and exhibits antitumor effects in human HCC cells. Therefore, this study examined the tumor-suppression efficiency of AE-SN integrated with a standard chemotherapeutic drug, namely, cisplatin or doxorubicin, in human HCC cells, namely, Hep3B and HepJ5. The results suggested that the integrated treatment with AE-SN-potentiated cisplatin and doxorubicin induced cytotoxicity through the cleavage of caspase-7 and accumulation of microtubule-associated protein-1 light chain-3 A-1B II LC-3 A-B II, which were associated with apoptotic and autophagic cell death, respectively, in both the Hep3B and HepJ5 cells. In conclusion, AE-SN can potentially be used in novel integrated chemotherapy with cisplatin or doxorubicin to treat HCC patients.





Author: Chien-Kai Wang, Yi-Feng Lin, Cheng-Jeng Tai, Chia-Wowi Wang, Yu-Jia Chang, Chen-Yen Choong, Chi-Shian Lin, Chen-Jei Tai, a

Source: https://www.hindawi.com/



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