Protective Effect of Flos Lonicerae against Experimental Gastric Ulcers in Rats: Mechanisms of Antioxidant and Anti-Inflammatory ActionReport as inadecuate

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Evidence-Based Complementary and Alternative Medicine - Volume 2014 2014, Article ID 596920, 11 pages -

Research Article

School of Pharmacy, Sungkyunkwan University, Seobu-ro 2066, Jangan-gu, Suwon, Gyeonggi-do 440-746, Republic of Korea

Nonclinical Team, Green Cross Corp., 107 Inyeon-ro 30 Beon-gil, Giheung-gu, Yongin, Gyeonggi-do 446-799, Republic of Korea

Green Cross Health Science Co., Ltd., 474 Dunchon-daero, Jungwon-gu, Seongnam, Gyeonggi-do 462-725, Republic of Korea

Received 22 August 2014; Revised 21 November 2014; Accepted 11 December 2014; Published 24 December 2014

Academic Editor: Cheorl-Ho Kim

Copyright © 2014 Jung-Woo Kang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Flos Lonicerae is one of the oldest and most commonly prescribed herbs in Eastern traditional medicine to treat various inflammatory diseases. In the present study, we investigated the effects of ethyl acetate fraction of Flos Lonicerae GC-7101 on experimental gastric ulcer models and its mechanisms of action in gastric ulcer healing. The pharmacological activity of GC-7101 was investigated in rats on HCl-EtOH, indomethacin, water immersion restraint stress induced acute gastric ulcer, and acetic-acid-induced subchronic gastric ulcer. To determine its gastroprotective mechanisms, gastric wall mucus secretion, mucosal PGE2, mucosal NO content, nuclear translocation of NF-κB, mRNA expression of inflammatory cytokines, lipid peroxidation and glutathione content, and superoxide dismutase and catalase activities were measured. GC-7101 significantly attenuated development of acute gastric ulcer and accelerated the healing of acetic-acid-induced subchronic gastric ulcer. In HCl-EtOH-induced gastric ulcer, GC-7101 markedly enhanced gastric wall mucus content which was accompanied by increased mucosal PGE2 and NO production. Furthermore, treatment of GC-7101 exhibited anti-inflammatory and antioxidant activities as evidenced by decreased myeloperoxidase activity, NF-κB translocation, inflammatory cytokines mRNA expression, and lipid peroxidation and increased glutathione content and superoxide dismutase and catalase activities. These results demonstrated that GC-7101 possesses strong antiulcerogenic effect by modulating oxidative stress and proinflammatory mediators.

Author: Jung-Woo Kang, Nari Yun, Hae-Jung Han, Jeom-Yong Kim, Joo-Young Kim, and Sun-Mee Lee



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