Tolerance in TCR-Cognate Antigen Double-Transgenic Mice Mediated by Incomplete Thymic Deletion and Peripheral Receptor DownregulationReport as inadecuate




Tolerance in TCR-Cognate Antigen Double-Transgenic Mice Mediated by Incomplete Thymic Deletion and Peripheral Receptor Downregulation - Download this document for free, or read online. Document in PDF available to download.

Developmental Immunology - Volume 4 1995, Issue 4, Pages 299-315



lnstitute of Molecular Biology and Biotechnology, Crete, Greece

Division of Molecular Immunology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom

MRC Clinical Sciences Centre, Royal Postgraduate Medical School, Hammersmith Hospital, London W12 ONN, United Kingdom

Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DU, United Kingdom

Received 7 July 1995; Accepted 6 September 1995

Copyright © 1995 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Influenza nucleoprotein NP-specific T-cell receptor transgenic mice F5 were crossedwith transgenic mice expressing the cognate antigenic protein under the control of the H-2K

promoter. Double-transgenic mice show negative selection of thymocytes at theCD4

8

TCR

to CD4

8

TCR

transition stage. A few CD8 T cells, however, escape clonaldeletion, and in the peripheral lymphoid organs of these mice, they exhibit low levels ofthe transgenic receptor and upregulated levels of the CD44 memory marker. Such cells donot proliferate upon exposure to antigen stimulation in vivo or ex vivo, however, they candevelop low but detectable levels of antigen-specific cytotoxic function after stimulationin vitro in the presence of IL-2.





Author: Clio Mamalaki, Marianna Murdjeva, Mauro Tolaini, Trisha Norton, Phillip Chandler, Alain Townsend, Elizabeth Simpson, and Dimitris

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents