Detection of bovine respiratory syncytial virus in experimentally infected balb-c miceReport as inadecuate




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Abstract : The present study used an RT-nested-PCR and an immunohistochemistry assay to detect bovine respiratory syncytial virus in tissues from experimentally infected balb-c mice. As a first step, Chicken Embryo Related CER cell monolayers infected with the BRSV-25-BR strain isolated in Brazil were used for antigen production. Then, the infected lung and tracheal tissues of female balb-c mice were collected on 3, 5, 7 and 10 days post-infection and submitted to both techniques. Primers specific to F and G genes that amplify fragments of 481 bp and 371 bp, respectively, were used. The BRSV detection was not successful in all of the animals tested. The genomic fragment of the G gene from the organs of some infected mice on all analyzed post-infection days was amplified. However, in the RT-nested-PCR corresponding to the F gene, it was not possible to observe any amplified fragment. This was probably due to the higher sensitivity of the developed technique to amplify the fragment corresponding to the G gene compared to the F gene. Moreover, only three of the lungs collected five days post-infection were positive by immunohistochemistry. To the author-s knowledge, this is the first study reporting bovine respiratory syncytial virus detection in balb-c mice after experimental inoculation.

Keywords : bovine respiratory syncytial virus RT-nested-PCR immunohistochemistry mice experimental infection





Author: Renata Almeida Helena Domingues Lia Coswig Regina Celia Freitas d-Arce Rodrigo Franco de Carvalho Clarice Arns

Source: https://hal.archives-ouvertes.fr/



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