Survival, immune responses and tissue cyst production in outbred Swiss white and inbred CBA-Ca strains of mice experimentally infected with Neospora caninum tachyzoitesReport as inadecuate




Survival, immune responses and tissue cyst production in outbred Swiss white and inbred CBA-Ca strains of mice experimentally infected with Neospora caninum tachyzoites - Download this document for free, or read online. Document in PDF available to download.



Abstract : The present work compared inbred CBA-Ca and outbred Swiss white strains of mice for their capacity to cope with a Neospora caninum infection and to consistently produce tissue cysts. In each experiment Swiss white and CBA-Ca mice were given three different doses of NC-1 tachyzoites. Lymphoproliferative and humoral responses as well as cytokine production were evaluated eight weeks after infection PI whereas tissue cyst production and histopathology were assessed 4, 6 and 10 weeks PI in immunosuppressed mice. Tissue cysts were observed 10 weeks after infection only in CBA-Ca mice receiving the two highest inoculum doses. Furthermore this strain showed the highest specific lymphoproliferative response. A mixed cytokine response with elevated IFN-$\gamma$ and fairly low IL-4 and IL-10 secretion was recorded. In both strains, no lesions were observed in the tissues of infected mice. This study indicates that CBA-Ca female mice infected with 5 × 106 NC-1 tachyzoites represent a useful model for the study of specific maternal immune responses in pregnant animals.

keyword : Neospora caninum immune response resistance cyst mice





Author: Chantal Rettigner Thierry Leclipteux François De Meerschman Charles Focant Bertrand Losson

Source: https://hal.archives-ouvertes.fr/



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