Experimental infection of a US spike-insertion deletion porcine epidemic diarrhea virus in conventional nursing piglets and cross-protection to the original US PEDV infectionReport as inadecuate




Experimental infection of a US spike-insertion deletion porcine epidemic diarrhea virus in conventional nursing piglets and cross-protection to the original US PEDV infection - Download this document for free, or read online. Document in PDF available to download.

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Abstract : AbstractAlthough the original US porcine epidemic diarrhea virus PEDV was confirmed as highly virulent by multiple studies, the virulence of spike-insertion deletion S-INDEL PEDV strains is undefined.
In this study, 3–4 day-old conventional suckling piglets were inoculated with S-INDEL PEDV Iowa106 4 pig litters to study its virulence.
Two litters of age-matched piglets were inoculated with either the original US PEDV PC21A or mock as positive and negative controls, respectively.
Subsequently, all pigs were challenged with the original US PEDV PC21A on 21–29 days post-inoculation dpi to assess cross-protection.
All S-INDEL Iowa106- and the original US PC21A-inoculated piglets developed diarrhea.
However, the severity of clinical signs, mortality 0–75% and fecal PEDV RNA shedding titers varied among the four S-INDEL Iowa106-inoculated litters.
Compared with the original PC21A, piglets euthanized-died acutely from S-INDEL Iowa106 infection had relatively milder villous atrophy, lower antigen scores and more limited intestinal infection.
Two of four S-INDEL Iowa106-infected sows and the original PC21A-infected sow showed anorexia and watery diarrhea for 1–4 days.
After the original PC21A challenge, a subset 13-16 of S-INDEL Iowa106-inoculated piglets developed diarrhea, whereas all 5-5 and no 0-4 pigs in the mock and original PC21A-inoculated pigs had diarrhea, respectively.
Our results suggest that the virulence of S-INDEL PEDV Iowa106 was less than the original US PEDV PC21A in suckling pigs, with 100% morbidity and 18% 6-33 overall 0–75% mortality in suckling pigs depending on factors such as the sow’s health and lactation and the piglets’ birth weight.
Prior infection by S-INDEL Iowa106 provided partial cross-protection to piglets against the original PC21A challenge at 21–29 dpi.






Author: Chun-Ming Lin Thavamathi Annamalai Xinsheng Liu Xiang Gao Zhongyan Lu Mohamed El-Tholoth Hui Hu Linda J.
Saif - Qiuhong Wang -


Source: https://hal.archives-ouvertes.fr/



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