Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care UnitReport as inadecuate




Antifungal Susceptibility Patterns of Candida Species Recovered from Endotracheal Tube in an Intensive Care Unit - Download this document for free, or read online. Document in PDF available to download.

Advances in Medicine - Volume 2016 2016, Article ID 9242031, 6 pages -

Research Article

Department of Microbiology, Faculty of Science, Islamic Azad University, Varamin-Pishva Branch, Tehran, Iran

Department of Medical Parasitology and Mycology, Kurdistan University of Medical Sciences, P.O. Box 14155-6446, Sanandaj, Iran

Division of Molecular Biology, Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Department of Microbiology, Faculty of Science, Islamic Azad University, North Tehran Branch, Tehran, Iran

Department of Medical Mycology, Jondishapour University of Medical Sciences, Ahvaz, Iran

Department of Medical Mycology, Tarbiat Modares University, Tehran, Iran

Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Received 20 April 2016; Revised 11 June 2016; Accepted 15 June 2016

Academic Editor: Anastasia Kotanidou

Copyright © 2016 Elham Baghdadi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. Biofilms formed by Candida species which associated with drastically enhanced resistance against most antimicrobial agents. The aim of this study was to identify and determine the antifungal susceptibility pattern of Candida species isolated from endotracheal tubes from ICU patients. Methods. One hundred forty ICU patients with tracheal tubes who were intubated and mechanically ventilated were surveyed for endotracheal tube biofilms. Samples were processed for quantitative microbial culture. Yeast isolates were identified to the species level based on morphological characteristics and their identity was confirmed by PCR-RFLP. Antifungal susceptibility testing was determined according to CLSI document M27-A3. Results. Ninety-five strains of Candida were obtained from endotracheal tubes of which C. albicans ; 35.7% was the most frequently isolated species followed by other species which included C. glabrata ; 25.2%, C. parapsilosis ; 16.8%, C. tropicalis ; 12.6%, and C. krusei ; 9.4%. The resulting MIC90 for all Candida species were in increasing order as follows: caspofungin 0.5 μg-mL; amphotericin B 2 μg-mL; voriconazole 8.8 μg-mL; itraconazole 16 μg-mL; and fluconazole 64 μg-mL. Conclusion. Candida species recovered from endotracheal tube are the most susceptible to caspofungin.





Author: Elham Baghdadi, Sadegh Khodavaisy, Sassan Rezaie, Sara Abolghasem, Neda Kiasat, Zahra Salehi, Somayeh Sharifynia, and Farza

Source: https://www.hindawi.com/



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