Apoptosis in the lungs of pigs infected with porcine reproductive and respiratory syndrome virus and associations with the production of apoptogenic cytokinesReport as inadecuate




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Abstract : Apoptosis was studied in the lungs of pigs during an infection with a European strain of porcine reproductive and respiratory syndrome virus PRRSV and it was examined if cytokines were involved in the induction of apoptosis. Twenty-two 4- to 5-week-old gnotobiotic pigs were inoculated intranasally with 10$^{6.0}$ TCID$ {50}$ of the Lelystad virus and euthanised between 1 and 52 days post inoculation PI. The lungs and broncho-alveolar lavage BAL cells were assessed both for virus replication and apoptosis; BAL fluids were examined for interleukin IL-1, tumour necrosis factor-alpha and IL-10. Double-labellings were conducted to determine the relation between virus replication and apoptosis and to identify the apoptotic cells. Apoptosis occurred in both infected and non-infected cells. The percentages of infected cells, which were apoptotic, ranged between 9 and 39% in the lungs and between 13 and 30% in the BAL cells. The majority of apoptotic cells were non-infected. Non-infected apoptotic cells in the lungs were predominantly monocytes-macrophages, whereas those in the broncho-alveolar spaces were predominantly lymphocytes. The peak of apoptosis in the lungs at 14 days PI was preceded by a peak of IL-1 and IL-10 production at 9 days PI, suggesting a possible role of these cytokines in the induction of apoptosis in non-infected interstitial monocytes-macrophages. However, the latter hypothesis was not confirmed in vitro, since blood monocytes or alveolar macrophages did not undergo apoptosis after treatment with recombinant porcine IL-1 or IL-10.

Keywords : pig lung porcine reproductive and respiratory syndrome virus apoptosis cytokine





Author: Geoffrey Labarque Steven Van Gucht Hans Nauwynck Kristien Van Reeth Maurice Pensaert

Source: https://hal.archives-ouvertes.fr/



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