Humoral and cell-mediated immune responses of d-d histocompatible pigs against classical swine fever CSF virusReport as inadecuate




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Abstract : A better understanding of cell-mediated immune responses to classical swine fever virus CSFV is essential for the future development of improved vaccines. We analyzed the generation of cell-mediated and humoral immune responses in d-d histocompatible pigs following CSFV infection or vaccination. Viral infection induced high T cell responses with high primary and secondary CTL activity correlated with high IFN-$\gamma$ production, whereas vaccination with a live vaccine followed by infection mainly induced neutralizing antibody but low cell-mediated responses. Moreover, high IgG1 response was associated with high IFN-g response following infection whereas a weak IFN-$\gamma$ response was related to a good IgG2 response but a low IgG1 production. These data could reflect Th1-Th2-like balance of immune responses depending upon immunization protocols, which has not yet been described in the pig. T-cell responses to CSFV were evidenced by CSFV-specific CD25 upregulation on CD4$^{-}$CD8$^{+}$, but not on CD4$^{+}$CD8$^{-}$ cells, which further illustrated the importance of CTL responses after infection. Our results indicated that generation of cell-mediated immune responses was much higher following intranasal-oral CSFV infection than after intramuscular vaccination, which implies that the capacity of new CSFV vaccines to induce higher T-cell responses should be considered.

Keywords : immunity cytotoxicity CSFV d histocompatible pig





Author: Laurence Piriou Sylvie Chevallier Evelyne Hutet Bernard Charley Marie-Frédérique Le Potiera Emmanuel Albina

Source: https://hal.archives-ouvertes.fr/



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