The Over-expression of the β2 Catalytic Subunit of the Proteasome Decreases Homologous Recombination and Impairs DNA Double-Strand Break Repair in Human CellsReport as inadecuate




The Over-expression of the β2 Catalytic Subunit of the Proteasome Decreases Homologous Recombination and Impairs DNA Double-Strand Break Repair in Human Cells - Download this document for free, or read online. Document in PDF available to download.

Journal of Biomedicine and BiotechnologyVolume 2011 2011, Article ID 757960, 7 pages

Research ArticleLaboratorio di Terapia Genica e Molecolare, Istituto di Fisiologia Clinica CNR, Area della Ricerca CNR Via Moruzzi 1, 56125 Pisa, Italy

Received 23 December 2010; Accepted 16 March 2011

Academic Editor: Celina Janion

Copyright © 2011 Anita Collavoli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

By a human cDNA library screening, we have previously identified two sequences coding two different catalytic subunits of the proteasome which increase homologous recombination HR when overexpressed in the yeast Saccharomyces cerevisiae. Here, we investigated the effect of proteasome on spontaneous HR and DNA repair in human cells. To determine if the proteasome has a role in the occurrence of spontaneous HR in human cells, we overexpressed the β2 subunit of the proteasome in HeLa cells and determined the effect on intrachromosomal HR. Results showed that the overexpression of β2 subunit decreased HR in human cells without altering the cell proteasome activity and the Rad51p level. Moreover, exposure to MG132 that inhibits the proteasome activity reduced HR in human cells. We also found that the expression of the β2 subunit increases the sensitivity to the camptothecin that induces DNA double-strand break DSB. This suggests that the β2 subunit has an active role in HR and DSB repair but does not alter the intracellular level of the Rad51p.





Author: Anita Collavoli, Laura Comelli, Tiziana Cervelli, and Alvaro Galli

Source: https://www.hindawi.com/



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