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BioMed Research InternationalVolume 2014 2014, Article ID 757941, 11 pages

Research Article

Baqiyatallah University of Medical Sciences, Tehran, Iran

Young Researchers and Elites Club, Islamic Azad University, Shahrekord Branch, P.O. Box 166, Shahrekord, Iran

Intensive and Critical Care Nursing, Jiroft University of Medical Sciences, Jiroft, Iran

Scientific Association of Veterinary Office, College of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, P.O. Box 166, Shahrekord, Iran

Department of Gynecology, Imam Reza Hospital, AJA University of Medical Sciences, Tehran, Iran

Received 3 February 2014; Revised 18 May 2014; Accepted 9 June 2014; Published 12 August 2014

Academic Editor: Gundlapally S. Reddy

Copyright © 2014 Emad Yahaghi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

From a clinical and epidemiological perspective, it is important to know which genotypes and antibiotic resistance patterns are present in H. pylori strains isolated from salads and vegetables. Therefore, the present investigation was carried out to find this purpose. Three hundred eighty washed and unwashed vegetable samples and fifty commercial and traditional salad samples were collected from Isfahan, Iran. Samples were cultured and those found positive for H. pylori were analyzed using PCR. Antimicrobial susceptibility testing was performed using disk diffusion method. Seven out of 50 14% salad and 52 out of 380 13.68% vegetable samples harbored H. pylori. In addition, leek, lettuce, and cabbage were the most commonly contaminated samples 30%. The most prevalent virulence genes were oipA 86.44% and cagA 57.625. VacA s1a 37.28% and iceA1 47.45% were the most prevalent genotypes. Forty different genotypic combinations were recognized. S1a-cagA+-iceA1-oipA+ 33.89%, s1a-cagA+-iceA2-oipA 30.50%, and m1a-cagA+-iceA1-oipA+ 28.81% were the most prevalent combined genotypes. Bacterial strains had the highest levels of resistance against metronidazole 77.96%, amoxicillin 67.79%, and ampicillin 61.01%. High similarity in the genotyping pattern of H. pylori among vegetable and salad samples and human specimens suggests that vegetable and salads may be the sources of the bacteria.





Author: Emad Yahaghi, Faham Khamesipour, Fatemeh Mashayekhi, Farhad Safarpoor Dehkordi, Mohammad Hossein Sakhaei, Mojtaba Masoudimane

Source: https://www.hindawi.com/



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