Rt-pcr for detection of bovine parainfluenza virus type 3 bpiv-3 Report as inadecuate




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Amauri Braga Simonetti ; Paulo Michel Roehe ;Acta Scientiae Veterinariae 2008, 36 3

Author: Rodrigo de Almeida Vaucher

Source: http://www.redalyc.org/articulo.oa?id=289021806002


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Acta Scientiae Veterinariae ISSN: 1678-0345 ActaSciVet@ufrgs.br Universidade Federal do Rio Grande do Sul Brasil de Almeida Vaucher, Rodrigo; Braga Simonetti, Amauri; Roehe, Paulo Michel RT-PCR for detection of bovine parainfluenza virus type 3 (bPIV-3) Acta Scientiae Veterinariae, vol.
36, núm.
3, 2008, pp.
215-220 Universidade Federal do Rio Grande do Sul Porto Alegre, Brasil Available in: http:--www.redalyc.org-articulo.oa?id=289021806002 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Vaucher R.A., Simonetti A.B.
& Roehe P.M.
2008.
RT-PCR for detection of bovine parainfluenza virus type 3 (bPIV-3). Scientiae Veterinariae.
36(3): 215-220. Acta Scientiae Veterinariae.
36(3): 215-220,Acta 2008. ORIGINAL ARTICLE Pub.
788 ISSN 1678-0345 (Print) ISSN 1679-9216 (Online) RT-PCR for detection of bovine parainfluenza virus type 3 (bPIV-3) RT-PCR para detecção do vírus parainfluenza bovino tipo 3 (bPIV-3) Rodrigo de Almeida Vaucher1, Amauri Braga Simonetti2 & Paulo Michel Roehe2,3 ABSTRACT The RT-PCR technique has been frequentely used for detection of the human parainfluenza virus type 3 (hPIV-3) but the literature is scarce in relation to the bovine parainfluenza virus type 3 (bPIV-3).
The aim of this study was to describe a reverse transcriptase polymerase chain reaction (RT-PCR) for detection of bovine parainfluenza virus type 3 (bPIV-3) using degenerate oligonucleotides targeting a conserved region of hemagglutinin-neuraminidase (HN) gene.
Reference strain SF-4 and three different brazilian bPIV-3 isolates, besides five viral strains from different sources, were included in this study.
Viruses were cultured in MDBK cells under standard conditions.
Hemagglutination (HA) test was used for viral titration and a direct immunof...





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