Association and Linkage Disequilibrium Analyses of APOE Polymorphisms in AtherosclerosisReport as inadecuate




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Disease Markers - Volume 24 2008, Issue 2, Pages 65-72



Laboratory of Molecular Investigation, University Hospital Miguel Servet, Aragonian Health Sciences Institute, Zaragoza, Spain

Department of Biochemistry and Molecular and Cellular Biology, University of Zaragoza, Zaragoza, Spain

Division of Neurology, Hospital Virgen del Camino, Pamplona, Spain

Division of Internal Medicine, University Hospital Miguel Servet, Zaragoza, Spain

Received 7 January 2008; Accepted 7 January 2008

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background: Apolipoprotein E apo E plays a major role in lipid metabolism, and its genetic variations have been associated with cardiovascular risk. The objective of this study was to investigate the influence of the APOE promoter −491 A-T, −427 T-C and −219 G-T and coding region APOE ɛ2-ɛ3-ɛ4 polymorphisms in atherosclerosis disease by association and linkage disequilibrium analyses. Materials and methods: We analyzed these polymorphisms in a sample of 286 subjects with atherosclerosis disease: 153 subjects with atherothrombotic stroke ATS and 133 subjects with ischemic heart disease IHD; and in two control groups, 103 newborns and 114 elderly subjects. Results: The ɛ4 allele was associated with more severe carotid stenosis in the ATS group, being the percentages of ɛ4 carriers 26.7% and 11.4% for the higher and lower carotid stenosis groups, respectively p=0,066. The −491 T-T IHD subjects presented higher vessel scores than subjects A-A and A-T genotypes at that position p=0,041, and the frequencies of −2 5.1% versus 14.1%, p=0,060 and −427C 10.3% versus 24.4%, p=0,019 alleles were lower in IHD subjects with higher extent score versus lower extent score. The ɛ2 allele was in linkage disequilibrium with the −427C allele in all studied groups, and the −219T allele was associated with the ɛ4 allele in the IHD group. Conclusion: In summary, the ɛ2 allele was in linkage disequilibrium with the −427C allele in all studied groups, and only slight associations between the analyzed APOE polymorphisms in the promoter and in the coding region and carotid and coronary vascular disease have been observed.





Author: Marta Artieda, Alberto Gañán, Ana Cenarro, Ángel Luis García-Otín, Ivonne Jericó, Fernando Civeira, and Miguel Pocoví

Source: https://www.hindawi.com/



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