A pcr for differentiate between anaplasma marginale and a. centrale Report as inadecuate




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João Martins ; Aoi Masuda ; Adriana Seixas ; Itabajara da Silva Vaz Junior ;Acta Scientiae Veterinariae 2015, 43

Author: Ana Carolina Joazeiro

Source: http://www.redalyc.org/articulo.oa?id=289039763012


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Acta Scientiae Veterinariae ISSN: 1678-0345 ActaSciVet@ufrgs.br Universidade Federal do Rio Grande do Sul Brasil Joazeiro, Ana Carolina; Martins, João; Masuda, Aoi; Seixas, Adriana; da Silva Vaz Junior, Itabajara A PCR for Differentiate between Anaplasma marginale and A.
centrale Acta Scientiae Veterinariae, vol.
43, 2015, pp.
1-7 Universidade Federal do Rio Grande do Sul Porto Alegre, Brasil Available in: http:--www.redalyc.org-articulo.oa?id=289039763012 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Acta Scientiae Veterinariae, 2015.
43: 1270. RESEARCH ARTICLE Pub.
1270 ISSN 1679-9216 A PCR for Differentiate between Anaplasma marginale and A.
centrale Ana Carolina Joazeiro1,2, João Martins3, Aoi Masuda1, Adriana Seixas1,4 & Itabajara da Silva Vaz Junior1,2 ABSTRACT Background: Anaplasma marginale ssp.
centrale (A.
centrale) exhibits low pathogenicity and therefore is used as a live vaccine against bovine anaplasmosis in several countries.
During production of the vaccine, accidental contamination with Anaplasma marginale (A.
marginale) is a risk that can jeopardize the entire batch of vaccine. Due to limitation of microscopic examination to detect low levels of parasitaemia, the present study aims to standardize a polymerase chain reaction assay using primers for the msp4 gene of Anaplasma sp.
for detecting and differentiating with greater sensitivity and specificity the species of A.
centrale and A.
marginale in blood samples from experimentally infected cattle. Materials, Methods & Results: The DNA extraction was performed from frozen blood.
Erythrocytes infected with known A.
centrale, A.
marginale served as positive control and the erytrocytes infected with Babesia bovis and Babesia bigemina served as the negat...





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