In vitro differentiation of human skin-derived multipotent stromal cells into putative endothelial-like cellsReport as inadecuate




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BMC Developmental Biology

, 12:7

Cellular reprogramming

Abstract

BackgroundMultipotent stem cells have been successfully isolated from various tissues and are currently utilized for tissue-engineering and cell-based therapies. Among the many sources, skin has recently emerged as an attractive source for multipotent cells because of its abundance. Recent literature showed that skin stromal cells SSCs possess mesoderm lineage differentiation potential; however, the endothelial differentiation and angiogenic potential of SSC remains elusive. In our study, SSCs were isolated from human neonatal foreskin hNFSSCs and adult dermal skin hADSSCs using explants cultures and were compared with bone marrow hMSC-TERT and adipose tissue-derived mesenchymal stem cells hADMSCs for their potential differentiation into osteoblasts, adipocytes, and endothelial cells.

ResultsConcordant with previous studies, both MSCs and SSCs showed similar morphology, surface protein expression, and were able to differentiate into osteoblasts and adipocytes. Using an endothelial induction culture system combined with an in vitro matrigel angiogenesis assay, hNFSSCs and hADSSCs exhibited the highest tube-forming capability, which was similar to those formed by human umbilical vein endothelial cells HUVEC, with hNFSSCs forming the most tightly packed, longest, and largest diameter tubules among the three cell types. CD146 was highly expressed on hNFSSCs and HUVEC followed by hADSSCs, and hMSC-TERT, while its expression was almost absent on hADMSCs. Similarly, higher vascular density based on the expression of CD31, CD34, vWF, CD146 and SMA was observed in neonatal skin, followed by adult dermal skin and adipose tissue. Thus, our preliminary data indicated a plausible relationship between vascular densities, and the expression of CD146 on multipotent cells derived from those tissues.

ConclusionsOur data is the first to demonstrate that human dermal skin stromal cells can be differentiated into endothelial lineage. Hence, SSCs represents a novel source of stem-stromal cells for tissue regeneration and the vascularization of engineered tissues. Moreover, the CD146 investigations suggested that the microenvironmental niche might contribute to direct stromal cells multipotency toward certain lineages, which warrants further investigation.

AbbreviationsHUVEC Human umbilical vein endothelial cells

SSCs Skin stromal cells

hNFSSCs Human neonatal foreskin stromal cells

hADSSCs Human adult dermal skin stromal cells

hMSC-TERT Human mesenchymal stem cell-telomerase reverse transcriptase immortalized cell line

hADMSCs Human adipose derived mesenchymal stem cells

CD Cluster of differentiation

RT-PCR Real time-polymerase chain reaction.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-213X-12-7 contains supplementary material, which is available to authorized users.

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Author: Radhakrishnan Vishnubalaji - Muthurangan Manikandan - May Al-Nbaheen - Balamuthu Kadalmani - Abdullah Aldahmash - Nehad M A

Source: https://link.springer.com/article/10.1186/1471-213X-12-7



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