Molecular characterization of a mosaic locus in the genome of CandidatusLiberibacter asiaticusReport as inadecuate




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BMC Microbiology

, 12:18

Microbial genetics, genomics and proteomics

Abstract

BackgroundHuanglongbing HLB is a highly destructive disease of citrus production worldwide. -Candidatus Liberibacter asiaticus-, an unculturable alpha proteobacterium, is a putative pathogen of HLB. Information about the biology and strain diversity of -Ca. L. asiaticus- is currently limited, inhibiting the scope of HLB research and control.

ResultsA genomic region CLIBASIA 05640 to CLIBASIA 05650 of -Ca. L. asiaticus- showing hyper-sequence variation or locus mosaicism was identified and investigated using 262 bacterial strains 188 from China and 74 from Florida. Based on the characteristic electrophoretic profiles of PCR amplicons generated by a specific primer set, eight electrophoretic types E-types were identified, six E-types A, B, C, D, E, and F in China and four E-types A, C, G, and H in Florida. The -Ca. L. asiaticus- strains from China consisted predominately of E-type A 71.3% and E-type B 19.7%. In contrast, the -Ca. L. asiaticus- strains from Florida was predominated by E-type G 82.4%. Diversity of -Ca. L. asiaticus- in China was also evidenced. Strains from the high altitude Yunnan Province consisted of five E-types with E-type B being the majority 62.8%, whereas strains from the low altitude coastal Guangdong Province consisted of only two E-types with E-type A as the majority 97.0%. Sequence analyses revealed that variation of DNA amplicons was due to insertion-deletion events at CLIBASIA 05650 and the downstream intergenic region.

ConclusionsThis study demonstrated the genomic mosaicism of -Ca. L. asiaticus- resulted from active DNA insertion-deletion activities. Analyses of strain variation depicted the significant inter- and intra-continent diversity of -Ca. L. asiaticus-.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-12-18 contains supplementary material, which is available to authorized users.

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Author: Xuefeng Wang - Changyong Zhou - Xiaoling Deng - Huanan Su - Jianchi Chen

Source: https://link.springer.com/article/10.1186/1471-2180-12-18







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