Temporal transcriptome changes induced by MDV in mareks disease-resistant and -susceptible inbred chickensReport as inadecuate




Temporal transcriptome changes induced by MDV in mareks disease-resistant and -susceptible inbred chickens - Download this document for free, or read online. Document in PDF available to download.

BMC Genomics

, 12:501

First Online: 12 October 2011Received: 23 February 2011Accepted: 12 October 2011

Abstract

BackgroundMarek-s disease MD is a lymphoproliferative disease in chickens caused by Marek-s disease virus MDV and characterized by T cell lymphoma and infiltration of lymphoid cells into various organs such as liver, spleen, peripheral nerves and muscle. Resistance to MD and disease risk have long been thought to be influenced both by genetic and environmental factors, the combination of which contributes to the observed outcome in an individual. We hypothesize that after MDV infection, genes related to MD-resistance or -susceptibility may exhibit different trends in transcriptional activity in chicken lines having a varying degree of resistance to MD.

ResultsIn order to study the mechanisms of resistance and susceptibility to MD, we performed genome-wide temporal expression analysis in spleen tissues from MD-resistant line 63, susceptible line 72 and recombinant congenic strain M RCS-M that has a phenotype intermediate between lines 63 and 72 after MDV infection. Three time points of the MDV life cycle in chicken were selected for study: 5 days post infection dpi, 10dpi and 21dpi, representing the early cytolytic, latent and late cytolytic stages, respectively. We observed similar gene expression profiles at the three time points in line 63 and RCS-M chickens that are both different from line 72. Pathway analysis using Ingenuity Pathway Analysis IPA showed that MDV can broadly influence the chickens irrespective of whether they are resistant or susceptible to MD. However, some pathways like cardiac arrhythmia and cardiovascular disease were found to be affected only in line 72; while some networks related to cell-mediated immune response and antigen presentation were enriched only in line 63 and RCS-M. We identified 78 and 30 candidate genes associated with MD resistance, at 10 and 21dpi respectively, by considering genes having the same trend of expression change after MDV infection in lines 63 and RCS-M. On the other hand, by considering genes with the same trend of expression change after MDV infection in lines 72 and RCS-M, we identified 78 and 43 genes at 10 and 21dpi, respectively, which may be associated with MD-susceptibility.

ConclusionsBy testing temporal transcriptome changes using three representative chicken lines with different resistance to MD, we identified 108 candidate genes for MD-resistance and 121 candidate genes for MD-susceptibility over the three time points. Genes included in our resistance or susceptibility genes lists that are also involved in more than 5 biofunctions, such as CD8α, IL8, USP18, and CTLA4, are considered to be important genes involved in MD-resistance or -susceptibility. We were also able to identify several biofunctions related with immune response that we believe play an important role in MD-resistance.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-12-501 contains supplementary material, which is available to authorized users.

Ying Yu, Juan Luo, Apratim Mitra contributed equally to this work.

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Author: Ying Yu - Juan Luo - Apratim Mitra - Shuang Chang - Fei Tian - Huanmin Zhang - Ping Yuan - Huaijun Zhou - Jiuzhou Song

Source: https://link.springer.com/article/10.1186/1471-2164-12-501







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