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Science China Life Sciences

, Volume 54, Issue 6, pp 553–559

First Online: 26 June 2011Received: 12 March 2011Accepted: 23 April 2011

Abstract

Induced pluripotent stem iPS cell technology demonstrates that somatic cells can be reprogrammed to a pluripotent state by over-expressing four reprogramming factors. This technology has created an interest in deriving iPS cells from domesticated animals such as pigs, sheep and cattle. Moloney murine leukemia retrovirus vectors have been widely used to generate and study mouse iPS cells. However, this retrovirus system infects only mouse and rat cells, which limits its use in establishing iPS cells from other mammals. In our study, we demonstrate a novel retrovirus strategy to efficiently generate porcine iPS cells from embryonic fibroblasts. We transfected four human reprogramming factors Oct4, Sox2, Klf4 and Myc into fibroblasts in one step by using a VSV-G envelope-coated pantropic retrovirus that was easily packaged by GP2-293 cells. We established six embryonic stem ES-like cell lines in human ES cell medium supplemented with bFGF. Colonies showed a similar morphology to human ES cells with a high nuclei-cytoplasm ratio and phase-bright flat colonies. Porcine iPS cells could form embryoid bodies in vitro and differentiate into the three germ layers in vivo by forming teratomas in immunodeficient mice.

Keywordsinduced pluripotent stem cells Moloney murine leukemia retrovirus vectors embryoid body teratoma This article is published with open access at Springerlink.com

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Author: WeiMin Ruan - JianYong Han - Pin Li - SuYing Cao - Yang An - Bing Lim - Ning Li

Source: https://link.springer.com/article/10.1007/s11427-011-4179-5



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