Decrypting the H-NS-dependent regulatory cascade of acid stress resistance in Escherichia coliReport as inadecuate




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BMC Microbiology

, 10:273

First Online: 29 October 2010Received: 02 June 2010Accepted: 29 October 2010

Abstract

BackgroundH-NS regulates the acid stress resistance. The present study aimed to characterize the H-NS-dependent cascade governing the acid stress resistance pathways and to define the interplay between the different regulators.

ResultsWe combined mutational, phenotypic and gene expression analyses, to unravel the regulatory hierarchy in acid resistance involving H-NS, RcsB-P-GadE complex, HdfR, CadC, AdiY regulators, and DNA-binding assays to separate direct effects from indirect ones. RcsB-P-GadE regulatory complex, the general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways plays a central role in the regulatory cascade. However, H-NS also directly controls specific regulators of these pathways e.g. cadC and genes involved in general stress resistance hdeAB, hdeD, dps, adiY. Finally, we found that in addition to H-NS and RcsB, a third regulator, HdfR, inversely controls glutamate-dependent acid resistance pathway and motility.

ConclusionsH-NS lies near the top of the hierarchy orchestrating acid response centred on RcsB-P-GadE regulatory complex, the general direct regulator of glutamate-, arginine- and lysine-dependent acid resistance pathways.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-10-273 contains supplementary material, which is available to authorized users.

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Author: Evelyne Krin - Antoine Danchin - Olga Soutourina

Source: https://link.springer.com/article/10.1186/1471-2180-10-273



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