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BMC Genomics

, 11:438

Human and rodent genomics

Abstract

BackgroundThe pig genome is being sequenced and characterised under the auspices of the Swine Genome Sequencing Consortium. The sequencing strategy followed a hybrid approach combining hierarchical shotgun sequencing of BAC clones and whole genome shotgun sequencing.

ResultsAssemblies of the BAC clone derived genome sequence have been annotated using the Pre-Ensembl and Ensembl automated pipelines and made accessible through the Pre-Ensembl-Ensembl browsers. The current annotated genome assembly Sscrofa9 was released with Ensembl 56 in September 2009. A revised assembly Sscrofa10 is under construction and will incorporate whole genome shotgun sequence WGS data providing > 30× genome coverage. The WGS sequence, most of which comprise short Illumina-Solexa reads, were generated from DNA from the same single Duroc sow as the source of the BAC library from which clones were preferentially selected for sequencing. In accordance with the Bermuda and Fort Lauderdale agreements and the more recent Toronto Statement the data have been released into public sequence repositories Genbank-EMBL, NCBI-Ensembl trace repositories in a timely manner and in advance of publication.

ConclusionsIn this marker paper, the Swine Genome Sequencing Consortium SGSC sets outs its plans for analysis of the pig genome sequence, for the application and publication of the results.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-11-438 contains supplementary material, which is available to authorized users.

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Author: Alan L Archibald - Lars Bolund - Carol Churcher - Merete Fredholm - Martien AM Groenen - Barbara Harlizius - Kyung-Tai Le

Source: https://link.springer.com/article/10.1186/1471-2164-11-438







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