Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly Glossina morsitans morsitansReport as inadecuate




Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly Glossina morsitans morsitans - Download this document for free, or read online. Document in PDF available to download.

BMC Genomics

, 11:160

First Online: 09 March 2010Received: 26 October 2009Accepted: 09 March 2010

Abstract

BackgroundTsetse flies, vectors of African trypanosomes, undergo viviparous reproduction the deposition of live offspring. This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female-s lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse Glossina morsitans morsitans reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.

ResultsAnalysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries salivary gland, fat body-milk gland, and midgut identified 51 that are unique to the reproductive-immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects hypothetical conserved. The analysis also yielded seven putative proteins without significant homology to sequences present in the public database unknown genes. These proteins may represent unique functions associated with tsetse-s viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.

ConclusionThis paper identifies 51 putative proteins specific to a tsetse reproductive-immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.

AbbreviationsESTexpressed sequence tag

kbasekilobase

NRnonredundant

ntnucleotide

RT-PCRreverse transcriptase polymerase chain reaction

cDNAcopyDNA.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-11-160 contains supplementary material, which is available to authorized users.

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Author: Geoffrey M Attardo - José MC Ribeiro - Yineng Wu - Matthew Berriman - Serap Aksoy

Source: https://link.springer.com/article/10.1186/1471-2164-11-160



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