A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor XenograftsReport as inadecuate




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Journal of Biomarkers - Volume 2016 2016, Article ID 1274603, 11 pages -

Research Article

Department of Molecular Pathology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA

Cancer Pharmacology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA

Early Discovery, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA

Protein Sciences, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA

Received 1 March 2016; Accepted 21 April 2016

Academic Editor: Yuen-Li Chung

Copyright © 2016 Helen He et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Use of tyramide signal amplification TSA to detect autophagy biomarkers in formalin fixed and paraffin embedded FFPE xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demonstrate the feasibility of using high sensitivity TSA IHC assays to measure low abundant autophagy markers in FFPE xenograft tissue.





Author: Helen He, Yu Yang, Zhongmin Xiang, Lunyin Yu, Jouhara Chouitar, Jie Yu, Natalie Roy D’Amore, Ping Li, Zhi Li, Douglas B

Source: https://www.hindawi.com/



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