Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation TMDHReport as inadecuate




Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation TMDH - Download this document for free, or read online. Document in PDF available to download.

BMC Genomics

, 10:291

First Online: 01 July 2009Received: 15 January 2009Accepted: 01 July 2009

Abstract

BackgroundIn recent years there has been an increasing problem with Staphylococcus aureus strains that are resistant to treatment with existing antibiotics. An important starting point for the development of new antimicrobial drugs is the identification of -essential- genes that are important for bacterial survival and growth.

ResultsWe have developed a robust microarray and PCR-based method, Transposon-Mediated Differential Hybridisation TMDH, that uses novel bioinformatics to identify transposon inserts in genome-wide libraries. Following a microarray-based screen, genes lacking transposon inserts are re-tested using a PCR and sequencing-based approach. We carried out a TMDH analysis of the S. aureus genome using a large random mariner transposon library of around a million mutants, and identified a total of 351 S. aureus genes important for survival and growth in culture. A comparison with the essential gene list experimentally derived for Bacillus subtilis highlighted interesting differences in both pathways and individual genes.

ConclusionWe have determined the first comprehensive list of S. aureus essential genes. This should act as a useful starting point for the identification of potential targets for novel antimicrobial compounds. The TMDH methodology we have developed is generic and could be applied to identify essential genes in other bacterial pathogens.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-10-291 contains supplementary material, which is available to authorized users.

Roy R Chaudhuri, Andrew G Allen, Duncan J Maskell and Ian G Charles contributed equally to this work.

Download fulltext PDF



Author: Roy R Chaudhuri - Andrew G Allen - Paul J Owen - Gil Shalom - Karl Stone - Marcus Harrison - Timothy A Burgis - Michael

Source: https://link.springer.com/article/10.1186/1471-2164-10-291







Related documents