Identification of genes expressed in the hermaphrodite germ line of C. elegans using SAGEReport as inadecuate




Identification of genes expressed in the hermaphrodite germ line of C. elegans using SAGE - Download this document for free, or read online. Document in PDF available to download.

BMC Genomics

, 10:213

First Online: 09 May 2009Received: 02 December 2008Accepted: 09 May 2009

Abstract

BackgroundGerm cells must progress through elaborate developmental stages from an undifferentiated germ cell to a fully differentiated gamete. Some of these stages include exiting mitosis and entering meiosis, progressing through the various stages of meiotic prophase, adopting either a male sperm or female oocyte fate, and completing meiosis. Additionally, many of the factors needed to drive embryogenesis are synthesized in the germ line. To increase our understanding of the genes that might be necessary for the formation and function of the germ line, we have constructed a SAGE library from hand dissected C. elegans hermaphrodite gonads.

ResultsWe found that 4699 genes, roughly 21% of all known C. elegans genes, are expressed in the adult hermaphrodite germ line. Ribosomal genes are highly expressed in the germ line; roughly four fold above their expression levels in the soma. We further found that 1063 of the germline-expressed genes have enriched expression in the germ line as compared to the soma. A comparison of these 1063 germline-enriched genes with a similar list of genes prepared using microarrays revealed an overlap of 460 genes, mutually reinforcing the two lists. Additionally, we identified 603 germline-enriched genes, supported by in situ expression data, which were not previously identified. We also found >4 fold enrichment for RNA binding proteins in the germ line as compared to the soma.

ConclusionUsing multiple technological platforms provides a more complete picture of global gene expression patterns. Genes involved in RNA metabolism are expressed at a significantly higher level in the germ line than the soma, suggesting a stronger reliance on RNA metabolism for control of the expression of genes in the germ line. Additionally, the number and expression level of germ line expressed genes on the X chromosome is lower than expected based on a random distribution.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-10-213 contains supplementary material, which is available to authorized users.

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Author: Xin Wang - Yongjun Zhao - Kim Wong - Peter Ehlers - Yuji Kohara - Steven J Jones - Marco A Marra - Robert A Holt - Dona

Source: https://link.springer.com/article/10.1186/1471-2164-10-213



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