Upregulation of Toll-like receptor TLR expression and release of cytokines from P815 mast cells by GM-CSFReport as inadecuate




Upregulation of Toll-like receptor TLR expression and release of cytokines from P815 mast cells by GM-CSF - Download this document for free, or read online. Document in PDF available to download.

BMC Cell Biology

, 10:37

First Online: 09 May 2009Received: 21 January 2009Accepted: 09 May 2009

Abstract

BackgroudRecently, mast cells have been recognized to express several Toll-like receptors TLRs on their membrane surfaces, and granulocyte-macrophage colony-stimulating factor GM-CSF was reported to be able to alter expression of TLRs and cytokine production in neutrophils. However, whether GM-CSF modulates the expression of TLR and cytokine production in mast cells is not clear.

ResultsUsing flow cytometry and real time PCR techniques, we found that GM-CSF upregulated expression of TLR3 and TLR7 in P815 cells in a concentration dependent manner. GM-CSF also provoked approximately up to 2.4 and 2.3 fold increase in IL-13 and IL-6 release from P815 cells, respectively following 16 h incubation. GM-CSF induced IL-13 secretion, TLR3 and TLR7 expression appeared to be through activation of mitogen-activated protein kinase MAPK and phosphotidylinositol 3-kinase PI3K-Akt signaling pathways, whereas GM-CSF elicited IL-6 release seemed via Akt signaling pathway. At 10 ng-ml, GM-CSF significantly enhanced R-848-induced IL-6 release from P815 cells.

ConclusionThe ability of GM-CSF in modulation of expression of TLR3 and TLR7 in P815 mast cells and in stimulation of IL-13 and IL-6 release from P815 mast cells in vitro suggests that GM-CSF might play an important role in enhancing the innate immune responses of mast cell to viral infection

AbbreviationsTLRsToll-like receptors

GM-CSFgranulocyte-macrophage colonystimulating factor

MAPKmitogenactivated protein kinase

ERKextracellular signal-regulated kinase

STATsignal transducer and activators of transcription

RT-PCRreverse transcription-polymerase chain reaction.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2121-10-37 contains supplementary material, which is available to authorized users.

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Author: Haiwei Yang - Jifu Wei - Huiyun Zhang - Liyan Lin - Wei Zhang - Shaoheng He

Source: https://link.springer.com/article/10.1186/1471-2121-10-37



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