Evolutionary history of the UCP gene family: gene duplication and selectionReport as inadecuate




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BMC Evolutionary Biology

, 8:306

First Online: 03 November 2008Received: 27 March 2008Accepted: 03 November 2008

Abstract

BackgroundThe uncoupling protein UCP genes belong to the superfamily of electron transport carriers of the mitochondrial inner membrane. Members of the uncoupling protein family are involved in thermogenesis and determining the functional evolution of UCP genes is important to understand the evolution of thermo-regulation in vertebrates.

ResultsSequence similarity searches of genome and scaffold data identified homologues of UCP in eutherians, teleosts and the first squamates uncoupling proteins. Phylogenetic analysis was used to characterize the family evolutionary history by identifying two duplications early in vertebrate evolution and two losses in the avian lineage excluding duplications within a species, excluding the losses due to incompletely sequenced taxa and excluding the losses and duplications inferred through mismatch of species and gene trees. Estimates of synonymous and nonsynonymous substitution rates dN-dS and more complex branch and site models suggest that the duplication events were not associated with positive Darwinian selection and that the UCP is constrained by strong purifying selection except for a single site which has undergone positive Darwinian selection, demonstrating that the UCP gene family must be highly conserved.

ConclusionWe present a phylogeny describing the evolutionary history of the UCP gene family and show that the genes have evolved through duplications followed by purifying selection except for a single site in the mitochondrial matrix between the 5 and 6 α-helices which has undergone positive selection.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2148-8-306 contains supplementary material, which is available to authorized users.

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Author: Joseph Hughes - Francois Criscuolo

Source: https://link.springer.com/article/10.1186/1471-2148-8-306







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