Components of the ubiquitin-proteasome pathway compete for surfaces on Rad23 family proteinsReport as inadecuate




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BMC Biochemistry

, 9:4

First Online: 30 January 2008Received: 24 October 2007Accepted: 30 January 2008

Abstract

BackgroundThe delivery of ubiquitinated proteins to the proteasome for degradation is a key step in the regulation of the ubiquitin-proteasome pathway, yet the mechanisms underlying this step are not understood in detail. The Rad23 family of proteins is known to bind ubiquitinated proteins through its two ubiquitin-associated UBA domains, and may participate in the delivery of ubiquitinated proteins to the proteasome through docking via the Rad23 ubiquitin-like UBL domain.

ResultsIn this study, we investigate how the interaction between the UBL and UBA domains may modulate ubiquitin recognition and the delivery of ubiquitinated proteins to the proteasome by autoinhibition. We have explored a competitive binding model using specific mutations in the UBL domain. Disrupting the intramolecular UBL-UBA domain interactions in HHR23A indeed potentiates ubiquitin-binding. Additionally, the analogous surface on the Rad23 UBL domain overlaps with that required for interaction with both proteasomes and the ubiquitin ligase Ufd2. We have found that mutation of residues on this surface affects the ability of Rad23 to deliver ubiquitinated proteins to the proteasome.

ConclusionWe conclude that the competition of ubiquitin-proteasome pathway components for surfaces on Rad23 is important for the role of the Rad23 family proteins in proteasomal targeting.

AbbreviationsUBLubiquitin-like

UBAubiquitin-associated

Hispolyhistidine

Ububiquitin

HHR23Human Homolog of Rad23

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2091-9-4 contains supplementary material, which is available to authorized users.

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Author: Amanda M Goh - Kylie J Walters - Suzanne Elsasser - Rati Verma - Raymond J Deshaies - Daniel Finley - Peter M Howley

Source: https://link.springer.com/article/10.1186/1471-2091-9-4







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