Laser surgery of zebrafish Danio rerio embryos using femtosecond laser pulses: Optimal parameters for exogenous material delivery, and the lasers effect on short- and long-term developmentReport as inadecuate




Laser surgery of zebrafish Danio rerio embryos using femtosecond laser pulses: Optimal parameters for exogenous material delivery, and the lasers effect on short- and long-term development - Download this document for free, or read online. Document in PDF available to download.

BMC Biotechnology

, 8:7

First Online: 29 January 2008Received: 11 May 2007Accepted: 29 January 2008

Abstract

BackgroundFemtosecond fs laser pulses have recently received wide interest as an alternative tool for manipulating living biological systems. In various model organisms the excision of cellular components and the intracellular delivery of foreign exogenous materials have been reported. However, the effect of the applied fs laser pulses on cell viability and development has yet to be determined. Using the zebrafish Danio rerio as our animal model system, we address both the short- and long-term developmental changes following laser surgery on zebrafish embryonic cells.

ResultsAn exogenous fluorescent probe, fluorescein isothiocyanate FITC, was successfully introduced into blastomere cells and found to diffuse throughout all developing cells. Using the reported manipulation tool, we addressed whether the applied fs laser pulses induced any short- or long-term developmental effects in embryos reared to 2 and 7 days post-fertilization dpf. Using light microscopy and scanning electron microscopy we compared key developmental features of laser-manipulated and control samples, including the olfactory pit, dorsal, ventral and pectoral fins, notochord, pectoral fin buds, otic capsule, otic vesicle, neuromast patterning, and kinocilia of the olfactory pit rim and cristae of the lateral wall of the ear.

ConclusionIn our study, no significant differences in hatching rates and developmental morphologies were observed in laser-manipulated samples relative to controls. This tool represents an effective non-destructive technique for potential medical and biological applications.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6750-8-7 contains supplementary material, which is available to authorized users.

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Author: Vikram Kohli - Abdulhakem Y Elezzabi

Source: https://link.springer.com/article/10.1186/1472-6750-8-7



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