The FTO fat mass and obesity associated gene codes for a novel member of the non-heme dioxygenase superfamilyReport as inadecuate




The FTO fat mass and obesity associated gene codes for a novel member of the non-heme dioxygenase superfamily - Download this document for free, or read online. Document in PDF available to download.

BMC Biochemistry

, 8:23

First Online: 08 November 2007Received: 27 July 2007Accepted: 08 November 2007

Abstract

BackgroundGenetic variants in the FTO fat mass and obesity associated gene have been associated with an increased risk of obesity. However, the function of its protein product has not been experimentally studied and previously reported sequence similarity analyses suggested the absence of homologs in existing protein databases. Here, we present the first detailed computational analysis of the sequence and predicted structure of the protein encoded by FTO.

ResultsWe performed a sequence similarity search using the human FTO protein as query and then generated a profile using the multiple sequence alignment of the homologous sequences. Profile-to-sequence and profile-to-profile based comparisons identified remote homologs of the non-heme dioxygenase family.

ConclusionOur analysis suggests that human FTO is a member of the non-heme dioxygenase FeII- and 2-oxoglutarate-dependent dioxygenases superfamily. Amino acid conservation patterns support this hypothesis and indicate that both 2-oxoglutarate and iron should be important for FTO function. This computational prediction of the function of FTO should suggest further steps for its experimental characterization and help to formulate hypothesis about the mechanisms by which it relates to obesity in humans.

Abbreviations2OG2-oxoglutarate

AlkBAlkylated DNA repair protein

ESTsExpressed sequence tags

FGENESHFind Genes using HMM

FTOfat mass and obesity associated

HIFHypoxia-inducible factor

HMMsHidden Markov Models

JGIJoint Genome Institute

NBCINational Center for Biotechnology Information

NLSNuclear Localization Signal

SNPsSingle nucleotide polymorphisms

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2091-8-23 contains supplementary material, which is available to authorized users.

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Author: Luis Sanchez-Pulido - Miguel A Andrade-Navarro

Source: https://link.springer.com/article/10.1186/1471-2091-8-23



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