High degree of conservancy among secreted salivary gland proteins from two geographically distant Phlebotomus duboscqi sandflies populations Mali and KenyaReport as inadecuate




High degree of conservancy among secreted salivary gland proteins from two geographically distant Phlebotomus duboscqi sandflies populations Mali and Kenya - Download this document for free, or read online. Document in PDF available to download.

BMC Genomics

, 7:226

First Online: 04 September 2006Received: 14 July 2006Accepted: 04 September 2006

Abstract

BackgroundSalivary proteins from sandflies are potential targets for exploitation as vaccines to control Leishmania infection; in this work we tested the hypothesis that salivary proteins from geographically distant Phlebotomus duboscqi sandfly populations are highly divergent due to the pressure exerted by the host immune response. Salivary gland cDNA libraries were prepared from wild-caught P. duboscqi from Mali and recently colonised flies of the same species from Kenya.

ResultsTranscriptome and proteome analysis resulted in the identification of the most abundant salivary gland-secreted proteins. Orthologues of these salivary proteins were identified by phylogenetic tree analysis. Moreover, comparative analysis between the orthologues of these two different populations resulted in a high level of protein identity, including the predicted MHC class II T-cell epitopes from all these salivary proteins.

ConclusionThese data refute the hypothesis that salivary proteins from geographically distinct populations of the same Phlebotomus sandfly species are highly divergent. They also suggest the potential for using the same species-specific components in a potential vector saliva-based vaccine.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-7-226 contains supplementary material, which is available to authorized users.

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Author: Hirotomo Kato - Jennifer M Anderson - Shaden Kamhawi - Fabiano Oliveira - Phillip G Lawyer - Van My Pham - Constance Sou

Source: https://link.springer.com/article/10.1186/1471-2164-7-226







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