Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus-insect cell system significantly enhanced by suspension cultureReport as inadecuate




Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus-insect cell system significantly enhanced by suspension culture - Download this document for free, or read online. Document in PDF available to download.

BMC Microbiology

, 6:16

First Online: 24 February 2006Received: 19 August 2005Accepted: 24 February 2006

Abstract

BackgroundPrevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.

ResultsFor vaccine production, hemagglutinin HA1 from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg-l, compared to 6 mg-l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein.

ConclusionThus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-6-16 contains supplementary material, which is available to authorized users.

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Author: Nitar Nwe - Qigai He - Sudarat Damrongwatanapokin - Qingyun Du - Ivanus Manopo - Yukol Limlamthong - Beau James Fenner - L

Source: https://link.springer.com/article/10.1186/1471-2180-6-16



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