Molecular dating of caprines using ancient DNA sequences of Myotragus balearicus, an extinct endemic Balearic mammalReport as inadecuate




Molecular dating of caprines using ancient DNA sequences of Myotragus balearicus, an extinct endemic Balearic mammal - Download this document for free, or read online. Document in PDF available to download.

BMC Evolutionary Biology

, 5:70

First Online: 06 December 2005Received: 11 June 2005Accepted: 06 December 2005

Abstract

BackgroundMyotragus balearicus was an endemic bovid from the Balearic Islands Western Mediterranean that became extinct around 6,000-4,000 years ago. The Myotragus evolutionary lineage became isolated in the islands most probably at the end of the Messinian crisis, when the desiccation of the Mediterranean ended, in a geological date established at 5.35 Mya. Thus, the sequences of Myotragus could be very valuable for calibrating the mammalian mitochondrial DNA clock and, in particular, the tree of the Caprinae subfamily, to which Myotragus belongs.

ResultsWe have retrieved the complete mitochondrial cytochrome b gene 1,143 base pairs, plus fragments of the mitochondrial 12S gene and the nuclear 28S rDNA multi-copy gene from a well preserved Myotragus subfossil bone. The best resolved phylogenetic trees, obtained with the cytochrome b gene, placed Myotragus in a position basal to the Ovis group. Using the calibration provided by the isolation of Balearic Islands, we calculated that the initial radiation of caprines can be dated at 6.2 ± 0.4 Mya. In addition, alpine and southern chamois, considered until recently the same species, split around 1.6 ± 0.3 Mya, indicating that the two chamois species have been separated much longer than previously thought.

ConclusionSince there are almost no extant endemic mammals in Mediterranean islands, the sequence of the extinct Balearic endemic Myotragus has been crucial for allowing us to use the Messinian crisis calibration point for dating the caprines phylogenetic tree.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2148-5-70 contains supplementary material, which is available to authorized users.

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Author: Carles Lalueza-Fox - Jose Castresana - Lourdes Sampietro - Tomàs Marquès-Bonet - Josep Antoni Alcover - Jaume Bertranpeti

Source: https://link.springer.com/article/10.1186/1471-2148-5-70







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