Detection of target DNA using fluorescent cationic polymer and peptide nucleic acid probes on solid supportReport as inadecuate




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BMC Biotechnology

, 5:10

First Online: 25 April 2005Received: 17 August 2004Accepted: 25 April 2005

Abstract

BackgroundNucleic acids detection using microarrays requires labelling of target nucleic acids with fluorophores or other reporter molecules prior to hybridization.

ResultsUsing surface-bound peptide nucleic acids PNA probes and soluble fluorescent cationic polythiophenes, we show a simple and sensitive electrostatic approach to detect and identify unlabelled target nucleic acid on microarray.

ConclusionThis simple methodology opens exciting possibilities for applied genetic analysis for the diagnosis of infections, identification of genetic mutations, and forensic inquiries. This electrostatic strategy could also be used with other nucleic acid detection methods such as electrochemistry, silver staining, metallization, quantum dots, or electrochemical dyes.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6750-5-10 contains supplementary material, which is available to authorized users.

Frédéric R Raymond, Hoang-Anh Ho contributed equally to this work.

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Author: Frédéric R Raymond - Hoang-Anh Ho - Régis Peytavi - Luc Bissonnette - Maurice Boissinot - François J Picard - Mario L

Source: https://link.springer.com/article/10.1186/1472-6750-5-10



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